Neurochemical Research

, Volume 37, Issue 3, pp 503–511

Phospho-Rb Mediating Cell Cycle Reentry Induces Early Apoptosis Following Oxygen–Glucose Deprivation in Rat Cortical Neurons

  • Ying Yu
  • Qing-Guo Ren
  • Zhao-Hui Zhang
  • Ke Zhou
  • Zhi-Yuan Yu
  • Xiang Luo
  • Wei Wang
Original Paper

DOI: 10.1007/s11064-011-0636-6

Cite this article as:
Yu, Y., Ren, QG., Zhang, ZH. et al. Neurochem Res (2012) 37: 503. doi:10.1007/s11064-011-0636-6

Abstract

The aim of this study was to investigate the relationship between cell cycle reentry and apoptosis in cultured cortical neurons following oxygen–glucose deprivation (OGD). We found that the percentage of neurons with BrdU uptake, TUNEL staining, and colocalized BrdU uptake and TUNEL staining was increased relative to control 6, 12 and 24 h after 1 h of OGD. The number of neurons with colocalized BrdU and TUNEL staining was decreased relative to the number of TUNEL-positive neurons at 24 h. The expression of phosphorylated retinoblastoma protein (phospho-Rb) was significantly increased 6, 12 and 24 h after OGD, parallel with the changes in BrdU uptake. Phospho-Rb and TUNEL staining were colocalized in neurons 6 and 12 h after OGD. This colocalization was strikingly decreased 24 h after OGD. Treatment with the cyclin-dependent kinase inhibitor roscovitine (100 μM) decreased the expression of phospho-Rb and reduced neuronal apoptosis in vitro. These results demonstrated that attempted cell cycle reentry with phosphorylation of Rb induce early apoptosis in neurons after OGD and there must be other mechanisms involved in the later stages of neuronal apoptosis besides cell cycle reentry. Phosphoralated Rb may be an important factor which closely associates aberrant cell cycle reentry with the early stages of neuronal apoptosis following ischemia/hypoxia in vitro, and pharmacological interventions for neuroprotection may be useful directed at this keypoint.

Keywords

Oxygen–glucose deprivation Neuron Cell cycle reentry Early apoptosis Phospho-Rb 

Supplementary material

11064_2011_636_MOESM1_ESM.docx (55 kb)
Supplementary material 1 (DOCX 54 kb)

Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Ying Yu
    • 1
  • Qing-Guo Ren
    • 3
  • Zhao-Hui Zhang
    • 1
  • Ke Zhou
    • 1
  • Zhi-Yuan Yu
    • 2
  • Xiang Luo
    • 2
  • Wei Wang
    • 2
  1. 1.Department of NeurologyRenmin Hospital of Wuhan UniversityWuhanPeople’s Republic of China
  2. 2.Department of Neurology, Tongji Hospital, Tongji Medical CollegeHuazhong University of Science and TechnologyWuhanPeople’s Republic of China
  3. 3.Department of NeurologyZhongda Hospital and the Institution of Cerebral Vascular Disease of Southeast UniversityNanjingPeople’s Republic of China