Neurochemical Research

, Volume 37, Issue 2, pp 417–427

Mimosine-Induced Apoptosis in C6 Glioma Cells Requires the Release of Mitochondria-Derived Reactive Oxygen Species and p38, JNK Activation

Authors

    • Department of Biomedical Sciences, College of Life and Health SciencesChubu University
  • Keiko Murakami
    • Department of BiochemistryAichi Medical University School of Medicine
  • Qinghong Zhao
    • Department of SurgeryThe Second Affiliated Hospital of Nanjing Medical University
  • Baoling Wang
    • Department of SurgeryThe Second Affiliated Hospital of Nanjing Medical University
  • Hisao Seo
    • Department of Biomedical Sciences, College of Life and Health SciencesChubu University
  • Hitoshi Yamashita
    • Department of Biomedical Sciences, College of Life and Health SciencesChubu University
  • Xiaotao Li
    • Institute of Biomedical SciencesEast China Normal University
  • Takashi Iwamoto
    • Department of Biomedical Sciences, College of Life and Health SciencesChubu University
  • Masatoshi Ichihara
    • Department of Biomedical Sciences, College of Life and Health SciencesChubu University
  • Masataka Yoshino
    • Department of Food and Nutritional EnvironmentKinjo Gakuin University
Original Paper

DOI: 10.1007/s11064-011-0628-6

Cite this article as:
Qiao, S., Murakami, K., Zhao, Q. et al. Neurochem Res (2012) 37: 417. doi:10.1007/s11064-011-0628-6
  • 209 Views

Abstract

Growth-inhibitory effects of mimosine, a plant amino acid, on rat C6 glioma cells were analyzed. Mimosine markedly inhibited proliferation and induced apoptosis of C6 glioma cells in a dose- and time-dependent manner. Mimosine-mediated apoptosis was accompanied by promoting reactive oxygen species (ROS) generation in mitochondria, and by decreased mitochondrial membrane potential (Δψ), and release of cytochrome c from mitochondria, followed by caspase 3 activation. Furthermore, mimosine increased the phosphorylation level of c-Jun-N-terminal protein kinase and p38, which was the downstream effect of ROS accumulation. Mimosine was confirmed to show profound effects on apoptosis of C6 glioma cells by ROS-regulated mitochondria pathway, and these results bear on the hypothesized potential for mimosine as promising agents in the treatment of malignant gliomas.

Keywords

C6 glioma cellMimosineROSApoptosisMitochondria

Copyright information

© Springer Science+Business Media, LLC 2011