Neurochemical Research

, 36:2169

Quinoprotein Adducts Accumulate in the Substantia Nigra of Aged Rats and Correlate with Dopamine-Induced Toxicity in SH-SY5Y Cells

Original Paper

DOI: 10.1007/s11064-011-0541-z

Cite this article as:
Wang, N., Wang, Y., Yu, G. et al. Neurochem Res (2011) 36: 2169. doi:10.1007/s11064-011-0541-z

Abstract

Parkinson’s disease (PD) is an age-dependent neurodegenerative disorder characterized by dopaminergic neuron loss in substantia nigra. Previous studies have implicated a role of dopamine oxidation in PD. Dopamine oxidation leads to the formation of dopamine quinone, which generates reactive oxygen species and covalently modifies cysteinyl proteins to form quinoprotein adduct. We compared quinoprotein adduct formation and lipid peroxidation in different brain regions of young and old rats. We found a prominent age-dependent accumulation of quinoprotein adducts in the substantia nigra, while no significant change of lipid peroxidation was detected in any brain regions of 2- to 15-month old rats. To determine whether quinoprotein adduct formation correlates with dopamine-induced cytotoxicity, we analyzed dopamine treated SH-SY5Y cells and found a strong correlation between quinoprotein adduct formation and cytotoxicity. Together, our results indicate that quinoprotein adduct formation may play a role in the age-dependent selective vulnerability of dopaminergic neurons in PD.

Keywords

Parkinson diseaseDopamineDopamine quinoneQuinoprotein adductLipid peroxidationAging

Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  1. 1.School of Life ScienceEast China Normal UniversityShanghaiChina
  2. 2.Department of Molecular and Cellular BiochemistryOhio State UniversityColumbusUSA