Neurochemical Research

, Volume 36, Issue 11, pp 2002–2007

Comparison of Neurosphere-like Cell Clusters Derived from Dental Follicle Precursor Cells and Retinal Müller Cells

  • Hans Christian Beck
  • Jørgen Petersen
  • Oliver Felthaus
  • Gottfried Schmalz
  • Christian Morsczeck
Original Paper

DOI: 10.1007/s11064-011-0524-0

Cite this article as:
Beck, H.C., Petersen, J., Felthaus, O. et al. Neurochem Res (2011) 36: 2002. doi:10.1007/s11064-011-0524-0

Abstract

Unrelated cells such as dental follicle precursor cells (DFPCs) and retinal Müller cells (MCs) make spheres after cultivation in serum-replacement medium (SRM). Until today, the relation and molecular processes of sphere formation from different cell types remain undescribed. Thus in this study we compared proteomes of spheres derived from MCs and DFPCs. 73% of 676 identified proteins were similar expressed in both cell types and many of them are expressed in the brain (55%). Moreover proteins are overrepresented that are associated with pathways for neural diseases such as Huntington disease or Alzheimer disease. Interestingly up-regulated proteins in DFPCs are involved in the biosynthesis of glycosphingolipids. These lipids are components of gangliosides such as GD3, which is a novel neural stem cell marker. In conclusion spheres from different types of cells have highly similar proteomes. These proteomes probably show essential cellular processes in neurosphere-like cell clusters.

Keywords

Dental follicle cellsMüller cellsRetinal progenitor cellsNeurospheresProteomics

Supplementary material

11064_2011_524_MOESM1_ESM.doc (1.1 mb)
Supplementary material 1 (DOC 1130 kb)

Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Hans Christian Beck
    • 1
  • Jørgen Petersen
    • 1
  • Oliver Felthaus
    • 2
  • Gottfried Schmalz
    • 2
  • Christian Morsczeck
    • 2
  1. 1.Danish Technological InstituteKoldingDenmark
  2. 2.Department of Operative Dentistry and PeriodontologyUniversity of RegensburgRegensburgGermany