Neurochemical Research

, Volume 36, Issue 5, pp 894–903

Copper Accelerates Glycolytic Flux in Cultured Astrocytes

Authors

  • Ivo F. Scheiber
    • Center for Biomolecular Interactions BremenUniversity of Bremen
    • Center for Environmental Research and Sustainable Technology
    • Center for Biomolecular Interactions BremenUniversity of Bremen
    • Center for Environmental Research and Sustainable Technology
    • School of Psychology and PsychiatryMonash University
Original Paper

DOI: 10.1007/s11064-011-0419-0

Cite this article as:
Scheiber, I.F. & Dringen, R. Neurochem Res (2011) 36: 894. doi:10.1007/s11064-011-0419-0
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Abstract

Astrocyte-rich primary cultures were used to investigate the consequences of a copper exposure on the glucose metabolism of astrocytes. After application of CuCl2 (30 μM) the specific cellular copper content increased from initial 1.5 ± 0.2 nmol/mg to a steady state level of 7.9 ± 0.9 nmol/mg within about 12 h. The copper accumulation was accompanied by a significant increase in the extracellular lactate concentration. The stimulating effect of copper on the lactate production remained after removal of extracellular copper. Copper treatment accelerated the rates of both glucose consumption and lactate production by about 60%. The copper induced acceleration of glycolytic flux was prevented by inhibition of protein synthesis, and additive to the stimulation of glycolysis observed for inhibitors of respiration or prolyl hydroxylases. A copper induced stimulation of glycolytic flux in astrocytes could have severe consequences for the glucose metabolism of the brain in conditions of copper overload.

Keywords

AstrocytesCopperGlucoseHIFLactate

Copyright information

© Springer Science+Business Media, LLC 2011