Neurochemical Research

, Volume 36, Issue 1, pp 83–92

Early Regional Response of Apoptotic Activity in Newborn Piglet Brain Following Hypoxia and Ischemia

  • A. Pirzadeh
  • A. Mammen
  • J. Kubin
  • E. Reade
  • H. Liu
  • A. Mendoza
  • W. J. Greeley
  • D. F. Wilson
  • A. Pastuszko
ORIGINAL PAPER

DOI: 10.1007/s11064-010-0267-3

Cite this article as:
Pirzadeh, A., Mammen, A., Kubin, J. et al. Neurochem Res (2011) 36: 83. doi:10.1007/s11064-010-0267-3

Abstract

Responses of selected neuroregulatory proteins that promote (Caspase 3 and Bax) or inhibit (Bcl-2, high Bcl-2/Bax ratio) apoptotic cell death were measured in the brain of piglets subjected to precisely controlled hypoxic and ischemic insults: 1 h hypoxia (decreasing FiO2 from 21 to 6%) or ischemia (ligation of carotid arteries and hemorrhage), followed by 0, 2 and 4 h recovery with 21% FiO2. Protein expression was measured in cortex, hippocampus and striatum by Western blot. There were no significant differences in expression of Caspase-3 between sham operated, hypoxic and ischemic groups. There were significant regional differences in expression of Bcl-2 and Bax in response to hypoxia and ischemia. The changes in Bcl-2/Bax ratio were similar for hypoxia and ischemia except for striatum at zero time recovery, with ischemia giving lower ratios than hypoxia. The Bcl-2/Bax ratio was also lower for the striatum than for the other regions of the brain, suggesting this region is the more susceptible to apoptotic injury.

Keywords

BrainApoptosisHypoxiaIschemiaNewborn

Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • A. Pirzadeh
    • 1
  • A. Mammen
    • 1
  • J. Kubin
    • 2
  • E. Reade
    • 1
  • H. Liu
    • 2
  • A. Mendoza
    • 2
  • W. J. Greeley
    • 1
  • D. F. Wilson
    • 2
  • A. Pastuszko
    • 2
  1. 1.Department of Anesthesiology and Critical Care MedicineChildren’s Hospital of PhiladelphiaPhiladelphiaUSA
  2. 2.Department Biochemistry and Biophysics, School of MedicineUniversity of PennsylvaniaPhiladelphiaUSA