Neurochemical Research

, Volume 35, Issue 10, pp 1620–1627

Intranigral LPS Administration Produces Dopamine, Glutathione but not Behavioral Impairment in Comparison to MPTP and 6-OHDA Neurotoxin Models of Parkinson’s Disease

  • Deborah Ariza
  • Marcelo M. S. Lima
  • Camila G. Moreira
  • Patrícia A. Dombrowski
  • Thiago V. Avila
  • Alexandra Allemand
  • Daniel A. G. B Mendes
  • Claudio Da Cunha
  • Maria A. B. F. Vital
ORIGINAL PAPER

DOI: 10.1007/s11064-010-0222-3

Cite this article as:
Ariza, D., Lima, M.M.S., Moreira, C.G. et al. Neurochem Res (2010) 35: 1620. doi:10.1007/s11064-010-0222-3

Abstract

The current investigation compared intranigral lipopolysaccharide (LPS), 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) and 6-hydroxydopamine (6-OHDA) administrations, in the light of neurochemical, behavioral and endogenous antioxidant glutathione alterations. All the results were collected 1, 3 and 7 days after the lesions. LPS produced a delayed reduction of striatal dopamine, whereas homovanillic acid was drastically increased at the first time-point. Comparatively, MPTP promoted dopamine reduction 3 and 7 days with increase of homovanillic acid. Whilst, 6-OHDA generated initial increase of dopamine and homovanillic acid followed by subsequent decrease of this neurotransmitter accompanied by reductions of dopamine metabolites at the same periods. Furthermore, nigral glutathione demonstrated to be a far more sensitive target for LPS than for MPTP or 6-OHDA. Behavioral data indicated impairments induced by MPTP, 6-OHDA but not LPS. In conclusion, it is suggested that intranigral LPS can provide new insights about neuroinflammation, simulating features of the pre-motor phase of Parkinson’s disease.

Keywords

NeuroinflammationAnimal modelsGlutathioneLPS

Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Deborah Ariza
    • 1
  • Marcelo M. S. Lima
    • 1
  • Camila G. Moreira
    • 1
  • Patrícia A. Dombrowski
    • 1
  • Thiago V. Avila
    • 1
  • Alexandra Allemand
    • 1
  • Daniel A. G. B Mendes
    • 1
  • Claudio Da Cunha
    • 1
  • Maria A. B. F. Vital
    • 1
  1. 1.Departamento de FarmacologiaUniversidade Federal do Paraná, Setor de Ciências BiológicasCuritibaBrazil