Neurochemical Research

, Volume 35, Issue 9, pp 1323–1332

Protective Effects of the Synthetic Cannabinoids CP55,940 and JWH-015 on Rat Brain Mitochondria upon Paraquat Exposure

  • Carlos Velez-Pardo
  • Marlene Jimenez-Del-Rio
  • Silvia Lores-Arnaiz
  • Juanita Bustamante
Original Paper

DOI: 10.1007/s11064-010-0188-1

Cite this article as:
Velez-Pardo, C., Jimenez-Del-Rio, M., Lores-Arnaiz, S. et al. Neurochem Res (2010) 35: 1323. doi:10.1007/s11064-010-0188-1

Abstract

The effects of cannabinoids in mitochondria after acute oxidative stress insult are not fully established. We investigated the ability of CP55,940 and JWH-015 to scavenge reactive oxygen species and their effect on mitochondria permeability transition (MPT) in either a mitochondria-free superoxide anion generation system, intact rat brain mitochondria or in sub-mitochondrial particles (SMP) treated with paraquat (PQ). Oxygen consumption, mitochondrial membrane potential (Δψm) and MPT were determined as parameters of mitochondrial function. It is found that both cannabinoids effectively attenuate mitochondrial damage against PQ-induced oxidative stress by scavenging anion superoxide radical (O2∙−) and hydrogen peroxide (H2O2), maintaining Δψm and by avoiding Ca2+-induced mitochondrial swelling. Understanding the mechanistic action of cannabinoids on mitochondria might provide new insights into more effective therapeutic approaches for oxidative stress related disorders.

Keywords

CP55,940CannabinoidsJWH-015MitochondriaOxidative stressParaquat

Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Carlos Velez-Pardo
    • 1
  • Marlene Jimenez-Del-Rio
    • 1
  • Silvia Lores-Arnaiz
    • 2
  • Juanita Bustamante
    • 2
  1. 1.School of Medicine, Biomedical Research Institute, Neuroscience Research GroupUniversity of AntioquiaMedellinColombia
  2. 2.Laboratory of Free Radical Biology, School of Pharmacy and BiochemistryUniversity of Buenos AiresBuenos AiresArgentina