Neurochemical Research

, Volume 35, Issue 5, pp 811–819

The Fat-1 Mouse has Brain Docosahexaenoic Acid Levels Achievable Through Fish Oil Feeding

  • Sarah K. Orr
  • Jasmin Y. M. Tong
  • Jing X. Kang
  • David W. L. Ma
  • Richard P. Bazinet
Original Paper

DOI: 10.1007/s11064-010-0139-x

Cite this article as:
Orr, S.K., Tong, J.Y.M., Kang, J.X. et al. Neurochem Res (2010) 35: 811. doi:10.1007/s11064-010-0139-x

Abstract

Fat-1 transgenic mice endogenously convert n-6 to n-3 polyunsaturated fatty acids (PUFA). The aims of this study were to test whether a) fish oil feeding can attain similar brain n-3 PUFA levels as the fat-1 mouse, and b) fat-1 mouse brain docosahexaenoic acid (22:6n-3; DHA) levels can be potentiated by fish oil feeding. Fat-1 mice and their wildtype littermates consumed either a 10% safflower oil (SO) or a 2% fish oil and 8% safflower oil chow (FO). Brain total lipid and phospholipid fraction fatty acids were analyzed using GC-FID. Wildtype mice fed FO chow had similar brain levels of DHA as fat-1 mice fed SO chow. Fat-1 mice fed FO chow had similar brain n-3 PUFA levels as fat-1 mice fed SO chow. In conclusion, brain levels of DHA in the fat-1 mouse can be obtained by and were not further augmented with fish oil feeding.

Keywords

Fat-1MouseDocosahexaenoic acidBrainn-3 PUFAFish oil

Abbreviations

20:4n-6

Arachidonic acid

ChoGpl

Choline glycerophospholipids

DHA

Docosahexaenoic acid

EtnGpl

Ethanolamine glycerophospholipids

FO

2% fish oil, 8% safflower oil chow

18:1

n-9 oleic acid

16:0

Palmitic acid

PtdIns

Phosphatidylinositol

PtdSer

Phosphatidylserine

PUFA

Polyunsaturated fatty acid

SO

10% safflower oil chow

18:0

Stearic acid

Supplementary material

11064_2010_139_MOESM1_ESM.docx (19 kb)
Supplementary material 1 (DOCX 19 kb)

Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Sarah K. Orr
    • 1
  • Jasmin Y. M. Tong
    • 1
  • Jing X. Kang
    • 2
  • David W. L. Ma
    • 1
    • 3
  • Richard P. Bazinet
    • 1
  1. 1.Department of Nutritional Sciences, Faculty of MedicineUniversity of TorontoTorontoCanada
  2. 2.Department of MedicineHarvard Medical SchoolBostonUSA
  3. 3.Department of Human Health and Nutritional Sciences, College of Biological ScienceUniversity of GuelphGuelphCanada