Neurochemical Research

, Volume 35, Issue 5, pp 702–711

A Milk-Based Wolfberry Preparation Prevents Prenatal Stress-Induced Cognitive Impairment of Offspring Rats, and Inhibits Oxidative Damage and Mitochondrial Dysfunction In Vitro

  • Zhihui Feng
  • Haiqun Jia
  • Xuesen Li
  • Zhuanli Bai
  • Zhongbo Liu
  • Lijuan Sun
  • Zhongliang Zhu
  • Peter Bucheli
  • Olivier Ballèvre
  • Junkuan Wang
  • Jiankang Liu
Original Paper

DOI: 10.1007/s11064-010-0123-5

Cite this article as:
Feng, Z., Jia, H., Li, X. et al. Neurochem Res (2010) 35: 702. doi:10.1007/s11064-010-0123-5
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Abstract

Lycium barbarum (Fructus Lycii, Wolfberry, or Gouqi) belongs to the Solanaceae. The red-colored fruits of L. barbarum have been used for a long time as an ingredient in Chinese cuisine and brewing, and also in traditional Chinese herbal medicine for improving health. However, its effects on cognitive function have not been well studied. In the present study, prevention of a milk-based wolfberry preparation (WP) on cognitive dysfunction was tested in a prenatal stress model with rats and the antioxidant mechanism was tested by in vitro experiments. We found that prenatal stress caused a significant decrease in cognitive function (Morris water maze test) in female offspring. Pretreatment of the mother rats with WP significantly prevented the prenatal stress-induced cognitive dysfunction. In vitro studies showed that WP dose-dependently scavenged hydroxyl and superoxide radicals (determined by an electron spin resonance spectrometric assay), and inhibited FeCl2/ascorbic acid-induced dysfunction in brain tissue and tissue mitochondria, including increases in reactive oxygen species and lipid peroxidation and decreases in the activities of complex I, complex II, and glutamate cysteine ligase. These results suggest that dietary supplementation with WP may be an effective strategy for preventing the brain oxidative mitochondrial damage and cognitive dysfunction associated with prenatal stress.

Keywords

Hydroxyl and superoxide radicals Lycium barbarum (wolfberry) Mitochondrial dysfunction Oxidative damage Prenatal stress 

Abbreviations

DMPO

5, 5-dimethyl-pyrroline N-oxide

DTPA

Diethylene triamine pentaacetic acid

HPX

Hypoxanthine

LBP

Lycium barbarum polysaccharides

4-POBN

α-(4-Pyridyl-1-oxide)-N-t-butylnitrone

PRS

Prenatal restraint stress

ROS

Reactive oxygen species

WP

A milk-based wolfberry preparation

XOD

Xanthine oxidase

Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Zhihui Feng
    • 1
    • 2
  • Haiqun Jia
    • 2
  • Xuesen Li
    • 2
  • Zhuanli Bai
    • 1
  • Zhongbo Liu
    • 2
  • Lijuan Sun
    • 3
  • Zhongliang Zhu
    • 1
  • Peter Bucheli
    • 4
  • Olivier Ballèvre
    • 5
  • Junkuan Wang
    • 6
  • Jiankang Liu
    • 1
    • 3
    • 7
  1. 1.Institute of Mitochondrial Biology and Medicine, Department of Biological Science and Engineering, The Key Laboratory of Biomedical Information Engineering of Ministry of EducationXi’an Jiaotong University School of Life Science and TechnologyXi’anChina
  2. 2.Institute for Nutritional Science, Shanghai Institutes of Biological Sciences, Chinese Academy of SciencesShanghai and Graduate School of the Chinese Academy of SciencesBeijingChina
  3. 3.College of Sports and HealthEast China Normal UniversityShanghaiChina
  4. 4.Nestlé Product Technology CenterKonolfingenSwitzerland
  5. 5.Science & ResearchNestlé R&D CentreBeijingChina
  6. 6.Nestlé Research CentreLausanneSwitzerland
  7. 7.Graduate Center for ToxicologyUniversity of Kentucky College of MedicineLexingtonUSA

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