Neurochemical Research

, Volume 35, Issue 4, pp 628–635

Human Serotonin Transporter Expression During Megakaryocytic Differentiation of MEG-01 Cells

  • Gino Giannaccini
  • Laura Betti
  • Lionella Palego
  • Lara Schmid
  • Laura Fabbrini
  • Caterina Pelosini
  • Claudia Gargini
  • Ylenia Da Valle
  • Mario Lanza
  • Alessandro Marsili
  • Margherita Maffei
  • Ferruccio Santini
  • Paolo Vitti
  • Aldo Pinchera
  • Antonio Lucacchini
Original Paper

DOI: 10.1007/s11064-009-0112-8

Cite this article as:
Giannaccini, G., Betti, L., Palego, L. et al. Neurochem Res (2010) 35: 628. doi:10.1007/s11064-009-0112-8

Abstract

The serotonin (5-HT) transporter (SERT) has been found altered in platelets of patients with genetically complex disorders, including mood-anxiety, pain and eating disorders. In this study, we used cell cultures of platelet precursors as models of investigation on mechanisms of SERT regulation: SERT expression was appraised during megakaryocytic differentiation of human megakaryoblastic MEG-01 cells. Cells were cultured for 8 days with 10−7M 4-β-12-tetradecanoylphorbol-13-acetate (β-TPA) in the presence of 10% fetal bovine serum (FBS) and SERT was assessed by real time PCR, immunofluorescence microscopy, Western blot and [3H]5-HT re-uptake. Results revealed that SERT is present in control-untreated MEG-01 cells. β-TPA-differentiating MEG-01 cells showed a redistribution of SERT fluorescence, diffuse to cell bodies and blebs along with a 3-fold SERT mRNA increase and a moderate raise in SERT protein (1.5/1.4-fold) by immunoblot and re-uptake assays. In summary, we have shown herein that control megakaryoblasts express the SERT protein. SERT is modulated by differentiation events, implying that SERT density in platelets is under the control of megakaryocytopoiesis stages. Differentiation of MEG-01 cells can provide considerable insight into interactions between SERT genetics, transmitter-hormonal/homeostatic mechanisms and signaling pathways.

Keywords

Serotonin transporter (SERT)MEG-01 cell lineDifferentiation4-β-12-tetradecanoylphorbol-13-acetate (β-TPA)

Copyright information

© Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Gino Giannaccini
    • 1
  • Laura Betti
    • 1
  • Lionella Palego
    • 1
  • Lara Schmid
    • 1
  • Laura Fabbrini
    • 1
  • Caterina Pelosini
    • 2
  • Claudia Gargini
    • 1
  • Ylenia Da Valle
    • 1
  • Mario Lanza
    • 1
  • Alessandro Marsili
    • 2
  • Margherita Maffei
    • 2
  • Ferruccio Santini
    • 2
  • Paolo Vitti
    • 2
  • Aldo Pinchera
    • 2
  • Antonio Lucacchini
    • 1
  1. 1.Department of Psychiatry, Neurobiology, Pharmacology and BiotechnologyUniversity of PisaPisaItaly
  2. 2.Department of Endocrinology and Metabolism, Sections of Endocrinology and Metabolism, Environment and Endocrine and Nervous Systems High Technology, Center for the Study of the Effects of Harmful AgentsUniversity of PisaPisaItaly