Neurochemical Research

, Volume 35, Issue 3, pp 357–365

Effect of Purple Sweet Potato Anthocyanins on β-Amyloid-Mediated PC-12 Cells Death by Inhibition of Oxidative Stress

  • Junli Ye
  • Xiangjun Meng
  • Chunling Yan
  • Chunbo Wang
Original Paper

DOI: 10.1007/s11064-009-0063-0

Cite this article as:
Ye, J., Meng, X., Yan, C. et al. Neurochem Res (2010) 35: 357. doi:10.1007/s11064-009-0063-0

Abstract

Amyloid-beta peptide (Aβ) is known to induce the redox imbalance, mitochondrial dysfunction and caspase activation, resulting in neuronal cell death. Treatment with antioxidants provided a new therapeutic strategy for Alzheimer’s disease (AD) patients. Here we investigate the effects of purple sweet potato anthocyanins (PSPA), the known strong free radical scavengers, on Aβ toxicity in PC12 cells. The results showed that pretreatment of PC12 cells with PSPA reduced Aβ-induced toxicity, intracellular reactive oxygen species (ROS) generation and lipid peroxidation dose-dependently. In parallel, cell apoptosis triggered by Aβ characterized with the DNA fragmentation and caspase-3 activity were also inhibited by PSPA. The concentration of intracellular Ca2+ and membrane potential loss associated with cell apoptosis were attenuated by PSPA. These results suggested that PSPA could protect the PC-12 cell from Aβ-induced injury through the inhibition of oxidative damage, intracellular calcium influx, mitochondria dysfunction and ultimately inhibition of cell apoptosis. The present study indicates that PSPA may be a promising approach for the treatment of AD and other oxidative-stress-related neurodegenerative diseases.

Keywords

PSPAAmyloid β peptide (Aβ)Oxidative stressReactive oxygen species (ROS)ApoptosisPC12 cells

Abbreviations

PSPA

Purple sweet potato anthocyanins

β-Amyloid

AD

Alzheimer’s disease

PC12

Pheochromocytoma cells

DMSO

Dimethylsulfoxide

DCF-DA

Dichlorofluorescin diacetate

MDA

Malonyl dialdehyde

MTT

3(4,5-dimethylthiazol-2yl)2,5-diphenyl-2H-tetrazolium bromide

OD

Optical density

PBS

Phosphate-buffered saline

ROS

Reactive oxygen species

LPO

Lipid peroxidation

RFU

Relative fluorescence unit

[Ca2+]i

Changes in intracellular free calcium levels

Copyright information

© Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Junli Ye
    • 1
  • Xiangjun Meng
    • 2
  • Chunling Yan
    • 1
  • Chunbo Wang
    • 3
  1. 1.Department of PathophysiologyMedical College, Qingdao UniversityQingdaoChina
  2. 2.Department of Psychological ConsultationNo. 7 People’s Hospital of QingdaoQingdaoChina
  3. 3.Department of PharmacologyMedical College, Qingdao UniversityQingdaoChina