Neurochemical Research

, Volume 34, Issue 2, pp 205–214

Estrogen Effects on High-Affinity Choline Uptake in Primary Cultures of Rat Basal Forebrain

  • Katie M. Bennett
  • Courtney Hoelting
  • Christopher P. Martin
  • James Stoll
Original Paper

DOI: 10.1007/s11064-008-9746-1

Cite this article as:
Bennett, K.M., Hoelting, C., Martin, C.P. et al. Neurochem Res (2009) 34: 205. doi:10.1007/s11064-008-9746-1
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Abstract

Basal forebrain cholinergic neurons (BFCNs) degenerate in aging and Alzheimer’s disease. It has been proposed that estrogen can affect the survival and function of BFCNs. This study characterized primary rat BFCN cultures and investigated the effect of estrogen on high-affinity choline uptake (HACU). BFCNs were identified by immunoreactivity to the vesicular acetylcholine transporter (VAChT) and represented up to 5% of total cells. HACU was measured in living BFCN cultures and differentiated from low-affinity choline uptake by hemicholinium-3 (HC-3) inhibition. A HC-3 concentration curve showed that 0.3 μM HC-3, but not higher concentrations that inhibit LACU, could distinguish the two transport activities. 17-β-Estradiol treatment increased HACU in some culture preparations that contained non-neuronal cells. Elimination of dividing cells using antimitotic treatments resulted in a lack of estrogen effects on HACU. These results suggest that estrogen may have indirect effects on BFCNs that are mediated through non-neuronal cells.

Keywords

Alzheimer’s disease Basal forebrain cholinergic neurons Choline uptake Estrogen Nerve growth factor Antimitotics 

Abbreviations

AD

Alzheimer’s disease

BFCNs

Basal forebrain cholinergic neurons

ChAT

Choline acetyltransferase

HACU

High-affinity choline uptake

HC-3

Hemicholinium-3

LACU

Low-affinity choline uptake

NGF

Nerve growth factor

VAChT

Vesicular acetylcholine transporter

VAChT-IR

VAChT-immunoreactive

Copyright information

© Springer Science+Business Media, LLC 2008

Authors and Affiliations

  • Katie M. Bennett
    • 1
  • Courtney Hoelting
    • 1
  • Christopher P. Martin
    • 1
  • James Stoll
    • 1
  1. 1.Department of Pharmaceutical SciencesTexas Tech University Health Sciences Center, School of PharmacyAmarilloUSA

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