Neurochemical Research

, Volume 33, Issue 12, pp 2547–2555

A Differential Proteomic Approach Reveals an Evolutionary Conserved Regulation of Nme Proteins by Fe65 in C. elegans and Mouse

  • Francesco Napolitano
  • Fulvio D’Angelo
  • Marida Bimonte
  • Valeria Perrina
  • Chiara D’Ambrosio
  • Andrea Scaloni
  • Tommaso Russo
  • Nicola Zambrano
Original Paper

DOI: 10.1007/s11064-008-9683-z

Cite this article as:
Napolitano, F., D’Angelo, F., Bimonte, M. et al. Neurochem Res (2008) 33: 2547. doi:10.1007/s11064-008-9683-z

Abstract

The function of the APP–Fe65 complex is still not definitively understood. To address this point we studied the phenotype of Fe65 (feh-1) ablation, which results in severe developmental defects in C. elegans, including embryonic and larval arrests. To shed light on the complex phenotype of embryonic arrest, we undertook a systematic approach, aiming at the definition of the altered proteomic profile of feh-1 null worms. We defined a panel of 27 regulated proteins, 16 of which actually participating to embryonic development processes in the nematode. Protein spots corresponding to the products of the F25H2.5 gene, the nematode orthologue of mammalian Nm23/Nme gene family members, were consistently up-regulated in feh-1 −/− embryos. We observed similar up-regulation of Nme1 and Nme2 genes, both at the transcript and the protein levels, in the brain of Fe65 knock-out mice, thus highlighting the occurrence of evolutionary conserved mechanisms of Nme expression in nematodes and mammals.

Keywords

Alzheimer’s diseaseAnimal models2D-DIGEGene expression

Copyright information

© Springer Science+Business Media, LLC 2008

Authors and Affiliations

  • Francesco Napolitano
    • 1
    • 2
  • Fulvio D’Angelo
    • 1
    • 2
  • Marida Bimonte
    • 1
    • 2
  • Valeria Perrina
    • 1
    • 2
  • Chiara D’Ambrosio
    • 3
  • Andrea Scaloni
    • 3
  • Tommaso Russo
    • 1
    • 2
  • Nicola Zambrano
    • 1
    • 2
  1. 1.CEINGE Biotecnologie AvanzateNapoliItaly
  2. 2.Dipartimento di Biochimica e Biotecnologie MedicheUniversità degli Studi di Napoli Federico IINapoliItaly
  3. 3.Proteomics and Mass Spectrometry Laboratory, ISPAAMConsiglio Nazionale delle RicercheNapoliItaly