Neurochemical Research

, Volume 33, Issue 10, pp 2044–2053

Curcumin Protects Dopaminergic Neuron Against LPS Induced Neurotoxicity in Primary Rat Neuron/Glia Culture

  • Sufen Yang
  • Dan Zhang
  • Zhengqin Yang
  • Xiaoming Hu
  • Steven Qian
  • Jie Liu
  • Belinda Wilson
  • Michelle Block
  • Jau-Shyong Hong
Original Paper

DOI: 10.1007/s11064-008-9675-z

Cite this article as:
Yang, S., Zhang, D., Yang, Z. et al. Neurochem Res (2008) 33: 2044. doi:10.1007/s11064-008-9675-z

Abstract

Using primary rat mesencephalic neuron-glia cultures as an in vitro model of Parkinson’s disease (PD), we tested the effect of curcumin, a natural dietary pigment with well-known anti-inflammation effects, on dopaminergic (DA) degeneration. Curcumin pretreatment mitigated LPS-induced DA neurotoxicity in a concentration-dependent manner and curcumin post-treatment also showed protective effect. Microglia depletion abolished this protective effect of curcumin, indicating that microglia play an important role in this effect. Supportively, observation by immunocytochemistry staining using OX-42 antibody showed that curcumin treatment inhibited LPS-induced morphological change of microglia. Besides, LPS-induced production of many proinflammatory factors and their gene expressions decreased dramatically after curcumin treatment. Results also revealed that curcumin treatment decreased LPS-induced activation of two transcription factors—nuclear factors κB (NF-κB) and activator protein-1 (AP-1). Taken together, our study implicated that curcumin might be a potential preventive and therapeutic strategy for inflammation-related neurodegenerative diseases.

Keywords

CurcuminMicrogliaDopaminergic neuronNeuroprotection

Copyright information

© Springer Science+Business Media, LLC 2008

Authors and Affiliations

  • Sufen Yang
    • 1
  • Dan Zhang
    • 1
  • Zhengqin Yang
    • 2
  • Xiaoming Hu
    • 1
  • Steven Qian
    • 1
  • Jie Liu
    • 3
  • Belinda Wilson
    • 1
  • Michelle Block
    • 1
  • Jau-Shyong Hong
    • 1
  1. 1.Laboratory of Pharmacology and ChemistryNational Institute of Environmental, Health Sciences, National Institutes of HealthResearch Triangle ParkUSA
  2. 2.National Center for Toxicogenomics, NIEHS, National Institutes of Health, School of Pharmaceutical SciencesZhengzhou UniversityZhengzhouP.R. China
  3. 3.Inorganic Carcinogenesis, Laboratory of Comparative CarcinogenesisNCI at NIEHSResearch Triangle ParkUSA