Neurochemical Research

, Volume 32, Issue 12, pp 2006–2014

Temporal Profiles of Cytoskeletal Protein Loss following Traumatic Axonal Injury in Mice

  • Gulyeter Serbest
  • Matthew F. Burkhardt
  • Robert Siman
  • Ramesh Raghupathi
  • Kathryn E. Saatman
Original Paper

DOI: 10.1007/s11064-007-9318-9

Cite this article as:
Serbest, G., Burkhardt, M.F., Siman, R. et al. Neurochem Res (2007) 32: 2006. doi:10.1007/s11064-007-9318-9

Abstract

To examine the time course and relative extent of proteolysis of neurofilament and tubulin proteins after traumatic axonal injury (TAI), anesthetized mice were subjected to optic nerve stretch injury. Immunohistochemistry confirmed neurofilament accumulation within axonal swellings at 4, 24, and 72 h postinjury (n = 4 injured and 2 sham per time point). Immunoblotting of optic nerve homogenates (n = 5 injured and 1 sham at 0.5, 4, 24 or 72 h) revealed calpain-mediated spectrin proteolytic fragments after injury. Protein levels for NF68 progressively decreased from 0.5 h to 24 h postinjury, while NF200 and α-tubulin levels decreased acutely (0.5–4 h), with a secondary decline at 72 h postinjury. These data demonstrate that diffusely distributed TAI is associated not only with a localized accumulation of neurofilament proteins, but also significant decreases in total cytoskeletal protein levels which may be mediated, in part, by calpains. Protection of the axonal cytoskeleton represents a potential therapeutic target for axonal damage associated with injury or neurodegenerative diseases.

Keywords

CalpainCytoskeletonNeurofilamentOptic nerveSpectrinTubulinWhite matter

Copyright information

© Springer Science+Business Media, LLC 2007

Authors and Affiliations

  • Gulyeter Serbest
    • 1
  • Matthew F. Burkhardt
    • 4
  • Robert Siman
    • 2
  • Ramesh Raghupathi
    • 3
  • Kathryn E. Saatman
    • 4
  1. 1.Department of NeurosurgeryUniversity of PennsylvaniaPhiladelphiaUSA
  2. 2.Department of PharmacologyUniversity of PennsylvaniaPhiladelphiaUSA
  3. 3.Department of Neurobiology & AnatomyDrexel University College of MedicinePhiladelphiaUSA
  4. 4.Department of Physiology, Spinal Cord & Brain Injury Research CenterUniversity of KentuckyLexingtonUSA