Neurochemical Research

, Volume 32, Issue 8, pp 1322–1328

Effect of Resuscitation with 21% Oxygen and 100% Oxygen on NMDA Receptor Binding Characteristics Following Asphyxia in Newborn Piglets

  • David Joseph Hoffman
  • Eric Lombardini
  • Om Prakash Mishra
  • Maria Delivoria-Papadopoulos
Original Paper

DOI: 10.1007/s11064-007-9307-z

Cite this article as:
Hoffman, D.J., Lombardini, E., Mishra, O.P. et al. Neurochem Res (2007) 32: 1322. doi:10.1007/s11064-007-9307-z
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Abstract

The present study investigated the effect of reventilation with 21% and 100% oxygen following asphyxia in newborn piglets on NMDA receptor binding characteristics, Na+, K+-ATPase activity, and lipid peroxidation. After achieving a heart rate less than 60 beats per minute, asphyxiated piglets were reventilated with 21% oxygen or 100% oxygen. 3[H]MK-801 binding showed the Bmax in the 21% and 100% groups to be 1.53 ± 0.43 and 1.42 ± 0.35 pmol/mg protein (p = ns). Values for Kd were 4.56 ± 1.29 and 4.17 ± 1.05 nM (p = ns). Na+, K+-ATPase activity in the 21% and 100% groups were 23.5 ± 0.9 and 24.4 ± 3.9 μmol Pi/mg protein/h (p = ns). Conjugated dienes (0.05 ± 0.02 vs. 0.07 ± 0.03 μmol/g brain) and fluorescent compounds (0.54 ± 0.05 vs. 0.78 ±  0.19 μg quinine sulfate/g brain), were similar in both groups (p = ns). Though lipid peroxidation products trended higher in the 100% group, these data show that NMDA receptor binding and Na+, K+-ATPase activity were similar following reventilation with 21% or 100% oxygen after a single episode of mild asphyxia.

Keywords

HyperoxiaBrainNMDAResuscitationNewborn

Copyright information

© Springer Science+Business Media, LLC 2007

Authors and Affiliations

  • David Joseph Hoffman
    • 1
    • 2
    • 3
  • Eric Lombardini
    • 1
    • 2
  • Om Prakash Mishra
    • 1
    • 2
  • Maria Delivoria-Papadopoulos
    • 1
    • 2
  1. 1.Department of PediatricsDrexel University College of MedicinePhiladelphiaUSA
  2. 2.St. Christopher’s Hospital for ChildrenPhiladelphiaUSA
  3. 3.The Reading Hospital and Medical CenterWest ReadingUSA