Neurochemical Research

, Volume 32, Issue 7, pp 1129–1141

Angiogenic Expression Profile of Normal and Neurofibromin-Deficient Human Schwann Cells

Original Paper

DOI: 10.1007/s11064-007-9279-z

Cite this article as:
Thomas, S.L. & De Vries, G.H. Neurochem Res (2007) 32: 1129. doi:10.1007/s11064-007-9279-z

Abstract

Peripheral nerve sheath tumors from individuals with Neurofibromatosis Type 1 (NF1) are highly vascular and contain Schwann cells which are deficient in neurofibromin. This study examines the angiogenic expression profile of neurofibromin-deficient human Schwann cells relative to normal human Schwann cells, characterizing both pro-angiogenic and anti-angiogenic factors. Conditioned media from neurofibromin-deficient Schwann cell lines was pro-angiogenic as evidenced by its ability to stimulate endothelial cell proliferation and migration. Using gene array and protein array analysis, we found increased expression of pro-angiogenic factors and decreased expression of anti-angiogenic factors in neurofibromin-deficient Schwann cells relative to normal human Schwann cells. Neurofibromin-deficient Schwann cells also showed increased expression of several growth factor receptors and decreased expression of an integrin. We conclude that neurofibromin-deficient Schwann cells have dysregulated expression of pro-angiogenic factors, anti-angiogenic factors, growth factor receptors, and an integrin. These dysregulated molecules may contribute to the growth and progression of NF1 peripheral nerve sheath tumors.

Keywords

NeurofibromatosisNF1NeurofibromaMPNSTNeurofibrominSchwann cellAngiogenesisAnti-angiogenicPro-angiogenic

Copyright information

© Springer Science+Business Media, LLC 2007

Authors and Affiliations

  1. 1.Research ServiceEdward Hines Jr. V.A. HospitalHinesUSA
  2. 2.Neuroscience ProgramLoyola University Medical CenterMaywoodUSA
  3. 3.Department of Anatomy and Cell BiologyUniversity of Illinois ChicagoChicagoUSA