Neurochemical Research

, Volume 32, Issue 7, pp 1099–1112

Mapping of QTLs for Oral Alcohol Self-Administration in B6.C and B6.I Quasi-Congenic RQI Strains

  • Csaba Vadasz
  • Mariko Saito
  • Beatrix M. Gyetvai
  • Melinda Oros
  • Istvan Szakall
  • Krisztina M. Kovacs
  • Vidudala V. T. S. Prasad
  • Grant Morahan
  • Reka Toth
Original paper

DOI: 10.1007/s11064-006-9234-4

Cite this article as:
Vadasz, C., Saito, M., Gyetvai, B.M. et al. Neurochem Res (2007) 32: 1099. doi:10.1007/s11064-006-9234-4

Abstract

One strategy to identify neurochemical pathways of addiction is to map the relevant genes. In the present study we used 43 B6.C and 35 B6.I inbred RQI mouse strains, carrying <3% donor genome on C57BL/6ByJ background, for gene mapping. The strains were phenotyped for consumption of alcohol (12% v/v) in a two-bottle-choice paradigm, and genotyped for 396 microsatellite markers. The current mapping study extends our earlier experiment scanning five mouse chromosomes (Vadasz et al. (2000) Scanning of five chromosomes for alcohol consumption loci. Alcohol 22:25–34) to a whole-genome study, and discusses the differences and limitations. Data were analyzed with composite interval (CIM) and multiple interval (MIM) QTL mapping methods. CIM of B6.C strains detected significant QTLs on chrs. 6 and 12. A suggestive, but not significant, locus was detected in the B6.I strains on chr. 12. The best MIM model for B6.C strains confirmed one QTL on chr. 6 and one QTL on chr. 12, while the MIM model for the B6.I strains confirmed the suggestive locus on chr. 12. Some of the QTLs for alcohol consumption are new, while others confirm previously reported QTLs for alcohol preference, and alcohol acceptance.

Keywords

QTL mappingQuasi-congenic mouseRecombinant QTL introgressionEthanol consumptionAddiction

Copyright information

© Springer Science+Business Media, LLC 2006

Authors and Affiliations

  • Csaba Vadasz
    • 1
    • 2
  • Mariko Saito
    • 1
    • 2
  • Beatrix M. Gyetvai
    • 1
  • Melinda Oros
    • 1
  • Istvan Szakall
    • 1
  • Krisztina M. Kovacs
    • 1
  • Vidudala V. T. S. Prasad
    • 1
  • Grant Morahan
    • 3
  • Reka Toth
    • 1
  1. 1.Laboratory of Neurobehavioral GeneticsNathan S. Kline Institute for Psychiatric ResearchOrangeburgUSA
  2. 2.Department of PsychiatryNew York University Medical CenterNew YorkUSA
  3. 3.Diabetes Research CentreWestern Australian Institute for Medical ResearchPerthAustralia
  4. 4.Department of BiochemistryDebrecen University School of MedicineDebrecenHungary
  5. 5.Animal HealthBayer Hungaria Kft.BudapestHungary
  6. 6.Neurobiology Research LaboratoryVA Medical CenterKansas CityUSA