Neurochemical Research

, Volume 31, Issue 2, pp 287–295

Cellular Localisation of Adenylyl Cyclase: A Post-genome Perspective

  • Ferenc A. Antoni
  • Ulrich K. Wiegand
  • Jamie Black
  • James Simpson
ORIGINAL PAPER

DOI: 10.1007/s11064-005-9019-1

Cite this article as:
Antoni, F.A., Wiegand, U.K., Black, J. et al. Neurochem Res (2006) 31: 287. doi:10.1007/s11064-005-9019-1

Abstract

The intracellular messenger cAMP is essential for vital processes ranging from ovulation to cognition. There are 10 genes for adenylyl cyclase (AC), the biosynthetic enzyme of cAMP. Nine of these encode membrane-bound proteins and one gives rise to soluble AC. The understanding of the biological significance of this molecular diversity is incomplete. Membrane-bound ACs conform to the same structural blueprint but have markedly different regulatory characteristics. AC mRNAs are differentially distributed in the body suggesting non-redundant physiological functions. The subcellular localisation of AC isoforms has not been examined in detail. Here we discuss the current knowledge on the intracellular targeting of AC isoforms, and highlight the technical problems of AC detection, some of which appear to be caused by the poor quality-control of commercially supplied antibodies. The principal message is that intracellular targeting of ACs may be isoform-specific and also dependent on the cellular context of expression.

Keywords

Immunocytochemistry Affinity-isolation Western blots Protein paralogs Antibody specificity 

Copyright information

© Springer Science+Business Media, Inc. 2006

Authors and Affiliations

  • Ferenc A. Antoni
    • 1
    • 3
  • Ulrich K. Wiegand
    • 2
  • Jamie Black
    • 1
  • James Simpson
    • 1
  1. 1.Division of NeuroscienceUniversity of EdinburghScotlandUK
  2. 2.Membrane Biology GroupUniversity of EdinburghScotlandUK
  3. 3.Division of NeuroscienceUniversity of EdinburghScotlandUK