Neurochemical Research

, Volume 30, Issue 2, pp 215–223

Effects of L-mono Methyl-arginine, N-Acetyl-cysteine and Diphenyleniodonium on Free Radical Release in C6 Glial Cells Enriched in Hexacosenoic Acid

  • Antonella Di Biase
  • Rita Di Benedetto
  • Serafina Salvati
  • Lucilla Attorri
  • Fabiana Leonardi
  • Donatella Pietraforte
Article

DOI: 10.1007/s11064-004-2444-8

Cite this article as:
Biase, A.D., Benedetto, R.D., Salvati, S. et al. Neurochem Res (2005) 30: 215. doi:10.1007/s11064-004-2444-8

Previously, we have shown that C6 glial cells enriched in hexacosenoic acid (HA) incubated with oxidative stressors released higher amounts of nitric oxide (NO) products and superoxide (O2), compared to native C6 cells. In the present study, we examined the effects of pre-treatment with some of free radical release inhibitors. The aim was to determine the origin of the enhanced generation of NO and superoxide, and to test the possibility of preventing it. Pre-treatment with L-mono-methyl-arginine and N-acetyl-cysteine in oxidized low-density lipoprotein (ox-LDL) exposed HA cells, inhibited not only nitrite but also superoxide production suggesting that (O2) anion could partially derive from inducible NO synthase. We also observed that ox-LDL treatment of HA cells reduced the intracellular glutathione levels and activated extracellular signal-related kinases. Since this signalling is related to neurotoxic effect, our data substantiate the role of the free radicals in X-linked adrenoleukodystrophy pathogenesis, as HA cells have been used as an in vitro model for this disease.

KEY WORDS

adrenoleukodystrophy antioxidants nitrite oxidized low-density lipoprotein reduced glutathione superoxide 

Copyright information

© Springer Science+Business Media, Inc. 2005

Authors and Affiliations

  • Antonella Di Biase
    • 1
  • Rita Di Benedetto
    • 1
  • Serafina Salvati
    • 1
  • Lucilla Attorri
    • 1
  • Fabiana Leonardi
    • 1
  • Donatella Pietraforte
    • 2
  1. 1.Food science, nutrition and health divisionIstituto Superiore di SanitàRomeItaly
  2. 2.Cellular biology and neuroscienceIstituto Superiore di SanitàRomeItaly