, Volume 42, Issue 4, pp 258–264

Effects of Epiphyseal Proteins and Melatonin on Blood Biochemical Parameters of Fluoride-Intoxicated Rats


DOI: 10.1007/s11062-011-9158-8

Cite this article as:
Bharti, V.K. & Srivastava, R.S. Neurophysiology (2011) 42: 258. doi:10.1007/s11062-011-9158-8

We examined the effects of fluoride intoxication on certain blood plasma biochemical indices in rats. Fortyeight adult female Wistar rats weighing 123-142 g were divided into eight groups: two control groups (0 and 28 days) and six experimental groups, namely sham-injected animals (vehicle), injected with pineal proteins (PP) and melatonin (Mel), intoxicated with fluoride (F), and also F+PP and F+Mel groups. Fluoride (150 ppm, per os administration with drinking water), melatonin (10 mg/kg, i.p.), and PP (100 μg/kg, i.p.) were administered daily for 28 days. Blood samples were collected at the end of experiments to estimate plasma [Na+] and [K+], alkaline phosphatase (ALP) activity, and levels of glucose and proteins in different animal groups. The plasma [K+] and [Na+], and ALP activity were significantly (P < 0.05) elevated in F-treated animals, as compared with others. Administration of PP and Mel in F-treated rats caused significant (P < < 0.05) reduction of [Na+], [K+], and ALP levels. Interestingly, PP and Mel administrations resulted in noticeable (P < 0.05) increases in the plasma glucose level in F-intoxicated animals, as compared to other groups. These findings convincingly indicate that PP and Mel exert ameliorative effects on fluoride-induced adverse changes in certain biochemical parameters in rats.


fluorideoxidative stressplasma biochemical compositionmelatoninpineal proteinsalkaline phosphatase

Copyright information

© Springer Science+Business Media, Inc. 2011

Authors and Affiliations

  1. 1.Indian Veterinary Research InstituteIzatnagarIndia
  2. 2.Nutrition and Toxicology Laboratory, Defence Institute of High Altitude Research (DIHAR)DRDO, Ministry of DefenceLehIndia