Journal of Neuro-Oncology

, Volume 112, Issue 2, pp 191–197

Supra- and infratentorial pediatric ependymomas differ significantly in NeuN, p75 and GFAP expression

  • Christian Hagel
  • András Treszl
  • Julia Fehlert
  • Jonas Harder
  • Franziska von Haxthausen
  • Meike Kern
  • André O. von Bueren
  • Uwe Kordes
Laboratory Investigation

DOI: 10.1007/s11060-013-1062-1

Cite this article as:
Hagel, C., Treszl, A., Fehlert, J. et al. J Neurooncol (2013) 112: 191. doi:10.1007/s11060-013-1062-1

Abstract

Ependymomas comprise 8 % of all intracranial tumors in children <15 years. Recent studies revealed that some supratentorial ependymomas express neuronal antigens and that high expression of neurofilament protein light polypeptide (NEFL) correlates with better clinical outcome. We retrospectively analyzed an expanded panel of proteins in 6 supratentorial, 15 posterior fossa and 4 spinal pediatric ependymomas by immunohistochemistry. Expression of high and low affinity neurotrophin receptors TrkA (NTRK1) and p75 (NGFR), pan-neuronal markers NeuN (RBFOX3) and synaptophysin, radial glial marker SOX9, adhesion molecules CD56 (NCAM) and CD44, junctional protein connexin 43 (GJA1), glial fibrillary acidic protein (GFAP), epithelial membrane antigen and proliferation associated antigen Ki-67 were evaluated in a semi-quantitative or quantitative (Ki-67 and NeuN-index) fashion. We found p75 and NeuN to be expressed at significantly higher levels in supratentorial versus infratentorial tumors and GFAP to be expressed at significantly higher levels in infratentorial lesions. In conclusion, immunohistochemical expression of p75, NeuN and GFAP differed in ependymomas depending on tumor topography supporting the view of divergent cells of origin. However, because of the small sample size the results are of preliminary nature and replication in a larger cohort would be desirable.

Keywords

EpendymomaChildhoodGFAPEMAp75NeuNSox9

Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • Christian Hagel
    • 1
  • András Treszl
    • 2
  • Julia Fehlert
    • 1
  • Jonas Harder
    • 1
  • Franziska von Haxthausen
    • 1
  • Meike Kern
    • 1
  • André O. von Bueren
    • 3
  • Uwe Kordes
    • 3
  1. 1.Institute of Neuropathology, University Medical Center Hamburg-EppendorfHamburgGermany
  2. 2.Department of Medical Biometry and EpidemiologyUniversity Medical Center Hamburg-EppendorfHamburgGermany
  3. 3.Department of Pediatric Hematology and OncologyUniversity Medical Center Hamburg-EppendorfHamburgGermany