Journal of Neuro-Oncology

, Volume 105, Issue 3, pp 499–506

Preliminary use of differential scanning calorimetry of cerebrospinal fluid for the diagnosis of glioblastoma multiforme

  • Alexis A. Chagovetz
  • Randy L. Jensen
  • Larry Recht
  • Michael Glantz
  • Alexander M. Chagovetz
Laboratory Investigation - Human/Animal Tissue

DOI: 10.1007/s11060-011-0630-5

Cite this article as:
Chagovetz, A.A., Jensen, R.L., Recht, L. et al. J Neurooncol (2011) 105: 499. doi:10.1007/s11060-011-0630-5

Abstract

Thermal stability signatures of complex molecule interaction in biological fluids can be measured using a new approach called differential scanning calorimetry (DSC). The thermal stability of plasma proteome has been described previously as a method of producing a disease-specific “signature,” termed thermogram, in several neoplastic and autoimmune diseases. We describe the preliminary use of DSC performed on cerebrospinal fluid (CSF) as a diagnostic tool for the identification of patients with glioblastoma multiforme (GBM). Samples of CSF from nine patients with confirmed GBM were evaluated using DSC, and the thermogram signatures evaluated. These thermograms were compared with thermograms of CSF taken from patients with non-neoplastic conditions such as head trauma, hydrocephalus, or CSF leak. Further analysis was also performed on CSF from patients who had non-GBM neoplastic conditions such as carcinomatosis meningitis or central nervous system lymphoma or leukemia. The DSC thermograms of CSF of the patients with GBM were significantly different when compared with other neoplastic and non-neoplastic cases. The melting temperature of the major transition was shifted by 5°C, which makes it easily distinguishable from control cases. Our results are very preliminary, but it appears that the DSC of CSF has potential utility in diagnostics and monitoring disease progression in GBM patients.

Keywords

Proteome Differential scanning calorimetry Protein Cerebrospinal fluid Glioblastoma 

Copyright information

© Springer Science+Business Media, LLC. 2011

Authors and Affiliations

  • Alexis A. Chagovetz
    • 1
    • 3
  • Randy L. Jensen
    • 2
  • Larry Recht
    • 4
  • Michael Glantz
    • 5
  • Alexander M. Chagovetz
    • 1
    • 3
  1. 1.Department of NeurosurgeryUniversity of UtahSalt Lake CityUSA
  2. 2.Departments of Neurosurgery, Radiation Oncology, and Oncological Sciences, Huntsman Cancer Institute, Clinical Neuroscience CenterUniversity of UtahSalt Lake CityUSA
  3. 3.Salt Lake City Bioscience, IncSalt Lake CityUSA
  4. 4.Department of NeurologyStanford UniversityStanfordUSA
  5. 5.Department of NeurosurgeryPenn State Hershey Medical CenterHersheyUSA