Journal of Neuro-Oncology

, Volume 104, Issue 1, pp 45–53

Isolation and characterization of tumor stem-like cells from human meningiomas

  • Dueng-Yuan Hueng
  • Huey-Kang Sytwu
  • Shih-Ming Huang
  • Chen Chang
  • Hsin-I Ma
Laboratory Investigation - Human/Animal Tissue

DOI: 10.1007/s11060-010-0469-1

Cite this article as:
Hueng, DY., Sytwu, HK., Huang, SM. et al. J Neurooncol (2011) 104: 45. doi:10.1007/s11060-010-0469-1

Abstract

Recent advances in research have found that tumor stem-like cells are resistant to surgery, radiotherapy, and chemotherapy. Tumor stem-like cells play critical roles in tumor recurrence, angiogenesis, and invasion in malignant brain tumors. However, the identification of tumor stem-like cells in meningiomas has not been clarified. In this study, we identified the stem-like features of sphere-forming cells in human meningiomas. The results showed that meningioma stem-like cells possess the ability to form spheres in identical stem cell culture condition. These meningioma sphere cells (MgSCs) expressed progenitor cell marker, CD133, but not differentiated cell marker, epithelial membrane antigen (EMA) on immunofluorescence staining. Importantly, the mRNA expression of ABCG, and CD133 was higher in MgSCs than daughter meningioma adherent cells (MgACs). In addition, cells displayed chemotherapeutic resistance to vincristine more in MgSCs than MgACs. This phenomenon was also found in single cell form from dissociated spheres than MgACs. Moreover, MgSCs are more resistant to irradiation treatment than MgACs. Furthermore, MgSCs revealed high tumorigenicity in vivo following orthotopic inoculation into the brains of immunodeficient mice. The corresponding immunohistochemical (IHC) staining found that MgSCs are positive for EMA, vimentin, and CD133, consistent with IHC of primary human meningiomas. These findings have provided better understanding of meningioma cell biology and suggested that meningioma stem-like cells may serve as a novel target in therapeutic resistant meningiomas.

Keywords

CD133ChemoresistanceMeningiomaRadioresistanceSphereTumor stem-like cell

Copyright information

© Springer Science+Business Media, LLC. 2010

Authors and Affiliations

  • Dueng-Yuan Hueng
    • 1
    • 2
  • Huey-Kang Sytwu
    • 2
    • 3
  • Shih-Ming Huang
    • 4
  • Chen Chang
    • 5
  • Hsin-I Ma
    • 1
    • 2
  1. 1.Department of Neurological Surgery, Tri-Service General Hospital, Graduate Institute of Medical SciencesNational Defense Medical CenterTaipeiTaiwan, ROC
  2. 2.Graduate Institute of Medical SciencesNational Defense Medical CenterTaipeiTaiwan, ROC
  3. 3.Graduate Institute of Life SciencesNational Defense Medical CenterTaipeiTaiwan, ROC
  4. 4.Department of BiochemistryNational Defense Medical CenterTaipeiTaiwan, ROC
  5. 5.Institute of Biomedical ScienceAcademia SinicaTaipeiTaiwan, ROC