Journal of Neuro-Oncology

, Volume 102, Issue 1, pp 19–24

Let-7 microRNA inhibits the proliferation of human glioblastoma cells

  • Soon-Tae Lee
  • Kon Chu
  • Hyun-Jung Oh
  • Woo-Seok Im
  • Ji-Yeon Lim
  • Seung-Ki Kim
  • Cheol-Ki Park
  • Keun-Hwa Jung
  • Sang Kun Lee
  • Manho Kim
  • Jae-Kyu Roh
Laboratory Investigation - Human/Animal Tissue

DOI: 10.1007/s11060-010-0286-6

Cite this article as:
Lee, S., Chu, K., Oh, H. et al. J Neurooncol (2011) 102: 19. doi:10.1007/s11060-010-0286-6

Abstract

MicroRNAs (miRNAs) are small noncoding RNAs comprising 21–23 nucleotides that regulate gene expression by transcriptionally repressing their complementary mRNAs. In particular, let-7 miRNA has been postulated to function as a tumor suppressor in various cancer cells, but not yet in glioblastoma. In this study, we investigated the anti-tumorigenic effect of let-7 miRNA in glioblastoma cells. Human glioblastoma cells (U251 or U87 cells) were transfected with let-7 miRNA and assayed for in-vitro proliferation, migration, and in-vivo tumor formation. Transfection of let-7 miRNA reduced expression of pan-RAS, N-RAS, and K-RAS in the glioblastoma cells. Let-7 miRNA also reduced the in-vitro proliferation and migration of the cells, and reduced the sizes of the tumors produced after transplantation into nude mice. However, let-7 miRNA exerted no effect on the proliferation of normal human astrocytes. These results indicate that let-7 miRNA has an anti-tumorigenic effect on glioblastoma cells, and suggest possible use of let-7 miRNA for treating glioblastoma.

Keywords

Let-7miRNAGlioblastomaRasProliferation

Copyright information

© Springer Science+Business Media, LLC. 2010

Authors and Affiliations

  • Soon-Tae Lee
    • 1
  • Kon Chu
    • 1
  • Hyun-Jung Oh
    • 1
  • Woo-Seok Im
    • 1
  • Ji-Yeon Lim
    • 1
  • Seung-Ki Kim
    • 2
  • Cheol-Ki Park
    • 2
  • Keun-Hwa Jung
    • 1
  • Sang Kun Lee
    • 1
  • Manho Kim
    • 1
  • Jae-Kyu Roh
    • 1
  1. 1.Department of Neurology, Clinical Research InstituteSeoul National University HospitalSeoulSouth Korea
  2. 2.Division of Pediatric Neurosurgery, Department of NeurosurgerySeoul National University Children’s HospitalSeoulSouth Korea