Laboratory Investigation - Human/Animal Tissue

Journal of Neuro-Oncology

, Volume 98, Issue 1, pp 49-55

Spontaneous canine gliomas: overexpression of EGFR, PDGFRα and IGFBP2 demonstrated by tissue microarray immunophenotyping

  • Robert J. HigginsAffiliated withDepartment of Pathology, Microbiology and Immunology, School of Veterinary Medicine, University of California Davis Email author 
  • , Peter J. DickinsonAffiliated withDepartment of Surgical and Radiological Sciences, School of Veterinary Medicine, University of California Davis
  • , Richard A. LeCouteurAffiliated withDepartment of Surgical and Radiological Sciences, School of Veterinary Medicine, University of California Davis
  • , Andrew W. BollenAffiliated withDepartment of Pathology, School of Medicine, University of California
  • , Huamin WangAffiliated withDepartment of Pathology, The University of Texas M.D. Anderson Cancer Center
  • , Hua WangAffiliated withDepartment of GI Medical Oncology, The University of Texas M.D. Anderson Cancer Center
  • , Linda J. CorelyAffiliated withDepartment of Pathology, The University of Texas M.D. Anderson Cancer Center
  • , Lynnette M. MooreAffiliated withDepartment of Pathology, The University of Texas M.D. Anderson Cancer Center
  • , Wei ZangAffiliated withDepartment of Pathology, The University of Texas M.D. Anderson Cancer Center
    • , Gregory N. FullerAffiliated withDepartment of Pathology, The University of Texas M.D. Anderson Cancer Center

Rent the article at a discount

Rent now

* Final gross prices may vary according to local VAT.

Get Access

Abstract

Fifty-seven spontaneous canine gliomas were histologically classified and graded using the latest World Health Organization (WHO 2007) criteria for classification of human gliomas. A total of 19 canine astrocytomas were classified as follows: grade IV (GBM) n = 7; grade III n = 5; and grade II, n = 7. Thirty-eight oligodendrogliomas were classified as either grade III (anaplastic) n = 35 or low grade II n = 3. Tissue microarray (TMA) immunohistochemistry was used to evaluate tumor expression of EGFR, PDGFRa and IGFBP2, three key molecules of known pathophysiological importance in human gliomas. Findings were correlated with tumor classification and grade. Increased EGFR expression was demonstrated in 57% of GBMs, 40% of grade III and 28% of grade II astrocytomas. EGFR expression occurred in only 3% of grade III oligodendrogliomas. Increased expression of PDGFRα was demonstrated in 43% of GBMs, 20% of grade III, and 14% of grade II astrocytomas. In the oligodendroglioma series, 94% of grade III tumors overexpressed PDGFRα. IGFBP2 expression was detected in 71, 60 and 28% of GBMs, grade III and grade II astrocytomas respectively. IGFBP2 expression occurred in 48% of anaplastic and in 33% of low grade oligodendrogliomas. Expression of EGFR, PDGFRα or IGFBP2 was not detected in normal canine CNS control TMA cores. The incidence of overexpression of EGFR, PDGFRα and IGFBP2 in these canine gliomas closely parallels that in human tumors of similar type and grade. These findings support a role for the spontaneous canine glioma model in directed pathway-targeting therapeutic studies.

Keywords

Canine EGFR Glioma IGFBP2 Immunohistochemistry PDGFRα Tissue microarray