Journal of Neuro-Oncology

, 76:1

The Effects of Antisense AKT2 RNA on the Inhibition of Malignant Glioma Cell Growth in vitro and in vivo

  • Peiyu Pu
  • Chunsheng Kang
  • Jie Li
  • Hao Jiang
  • Jinquan Cheng
Laboratory Investigation

DOI: 10.1007/s11060-005-3029-3

Cite this article as:
Pu, P., Kang, C., Li, J. et al. J Neurooncol (2006) 76: 1. doi:10.1007/s11060-005-3029-3

Summary

The oncogenic role of AKT2 in the development of malignant gliomas was examined by using antisense approach. AKT2 expression was significantly inhibited in rat C6 glioma cells transfected with antisense AKT2 cDNA construct (LXSN–AS–AKT2). In addition, the transfected cells proliferated at a lowered level and apoptosis was induced. For in vivo studies, parental C6 cells and C6 cells transfected with LXSN–AS–AKT2 were implanted stereotactically into the right caudate nucleus of SD rats (control C6 group and transfected group). The rats bearing well-established C6 gliomas were treated with LXSN–AS–AKT2 DNA or LXSN (empty vector)-lipofectamine complexes intratumorally (treated group and control treated group). The mean survival of the rats of control C6 group and treated control group was 17.8±0.92 days and 17.5±1.10 days, respectively. The mean survival of the rats of transfected and treated group was significantly prolonged. MR images revealed distinct cerebral tumor foci in all of the control rats, whereas four rats in transfected group did not develop tumors and the tumor foci in five rats of treated group were regressed and disappeared. The expression of AKT2, PCNA, MMP2/9, and cyclin D were inhibited in the tumors of rats in transfected and treated groups while GFAP expression was increased. These results suggest that AKT pathway may play an important role in the development and progression of gliomas. Anti-AKT approach will open a new perspective for a targeted molecular therapy of malignant gliomas.

Keywords

antisense AKT2 apoptosis cell proliferation malignant gliomas 

Copyright information

© Springer 2006

Authors and Affiliations

  • Peiyu Pu
    • 1
  • Chunsheng Kang
    • 1
  • Jie Li
    • 1
  • Hao Jiang
    • 2
  • Jinquan Cheng
    • 3
  1. 1.Department of NeurosurgeryTianjin Medical University General Hospital, Laboratory of Neuro-Oncology, Tianjin Neurological InstituteTianjinPeople’s Republic of China
  2. 2.William T. Gossett Neurology Laboratories, Department of NeurologyHenry Ford Health SystemDetroitUSA
  3. 3.Department of Pathology, H Lee Moffitt Cancer CenterUniversity of South Florida College of MedicineTampaUSA
  4. 4.Department of NeurosurgeryTianjin Medical University General HospitalTianjinPeople’s Republic of China

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