Journal of Neuro-Oncology

, Volume 70, Issue 3, pp 309–317

Quantification of thrombospondin-1 secretion and expression of αvβ3 and α3β1 integrins and syndecan-1 as cell-surface receptors for thrombospondin-1 in malignant glioma cells

  • Hirofumi Naganuma
  • Eiji Satoh
  • Takayuki Asahara
  • Kenichi Amagasaki
  • Arata Watanabe
  • Hiroki Satoh
  • Katsuhiro Kuroda
  • Lei Zhang
  • Hideaki Nukui
Laboratory Investigation

DOI: 10.1007/s11060-004-9167-1

Cite this article as:
Naganuma, H., Satoh, E., Asahara, T. et al. J Neurooncol (2004) 70: 309. doi:10.1007/s11060-004-9167-1

Abstract

Malignant glioma cells secrete thrombospondin-1 (TSP-1) which participates in the motility of glioma cells, and binds to cell surface αvβ3 and α3β1 integrins, and syndecan-1. This study evaluated the amount of TSP-1 secretion from malignant glioma cells, and the expression of αvβ3 and α3β1 integrins, and syndecan-1. The amounts of TSP-1 in the supernatants from 10 malignant glioma cell lines and eight non-glioma malignant tumor cell lines were measured by enzyme-linked immunosorbent assay. Expression of αvβ3 and α3β1 integrins, and syndecan-1 were examined by flow cytometry. The amounts of TSP-1 secreted by malignant glioma cells were 43 to 2431 ng/1 × 106 cells/24 h (mean ± SD=626 ± 792). Seven of 10 glioma cell lines secreted more than 100 ng of TSP-1 and three of these cell lines secreted more than 1 μg. Seven of eight non-glioma cell lines secreted less than 100 0ng of TSP-1. All glioma cell lines expressed α3β1 integrin and syndecan-1, and seven of 10 glioma cell lines expressed αvβ3 integrin. Treatment of the glioma cell lines with TGF-β2 did not change the expression of αvβ3 integrin. These results suggest that malignant glioma cells secrete high levels of TSP-1, which may be important in the migration of glioma cells via interactions with αvβ3 and α3β1 integrins, and syndecan-1.

Keywords

αvβ3 integrinα3β1 integrininvasionmalignant gliomasyndecan-1thrombospondin-1

Copyright information

© Kluwer Academic Publishers 2004

Authors and Affiliations

  • Hirofumi Naganuma
    • 1
  • Eiji Satoh
    • 1
  • Takayuki Asahara
    • 1
  • Kenichi Amagasaki
    • 1
  • Arata Watanabe
    • 1
  • Hiroki Satoh
    • 1
  • Katsuhiro Kuroda
    • 1
  • Lei Zhang
    • 1
  • Hideaki Nukui
    • 1
  1. 1.Department of Neurosurgery, Faculty of MedicineUniversity of YamanashiTamaho-machi, Nakakoma-gun, YamanashiJapan