Journal of Neuro-Oncology

, Volume 74, Issue 2, pp 99–103

Radiation-Enhanced Vascular Endothelial Growth Factor (VEGF) Secretion in Glioblastoma Multiforme Cell Lines – A Clue to Radioresistance?

  • K. E. Hovinga
  • L. J. A. Stalpers
  • C. van Bree
  • M. Donker
  • J. J. C. Verhoeff
  • H. M. Rodermond
  • D. A. Bosch
  • W. R. van Furth
Laboratory Investigation

DOI: 10.1007/s11060-004-4204-7

Cite this article as:
Hovinga, K.E., Stalpers, L.J.A., van Bree, C. et al. J Neurooncol (2005) 74: 99. doi:10.1007/s11060-004-4204-7

Summary

Objective: Postoperative radiotherapy is standard treatment for patients with a glioblastoma multiforme (GBM). However, a GBM is radioresistant and almost always recurs, even after a high dose of radiation. A GBM is characterized by its extensive neo-angiogenesis, which can be attributed to the high levels of vascular endothelial growth factor (VEGF). The scope of this study is to investigate the VEGF secretion by GBM cells with different radiosensitivity after irradiation. Methods:Three human GBM cell lines (U251, U251-NG2 and U87) were irradiated with single doses of 0, 5, 10 and 20 Gy of γ-rays from a 137Cs source. VEGF levels in medium were measured by ELISA at 24, 48 and 72 h after radiation. Cell survival was measured by the XTT assay 7 days after irradiation. Results:Following single dose radiation, the VEGF levels showed a dose dependent increase in U251, U251-NG2 and U87 glioma cells. Both base-line and radiation-enhanced VEGF levels were about 10-fold higher in U87 compared to U251 and U251-NG2 cells. In addition, in the XTT assay, the U87 was more radioresistant than both U251 and U251-NG2 cell lines (dose modifying factor (DMF) = 1.6 and 1.7 resp). Conclusion:Irradiation enhanced VEGF secretion in all three tested glioma cell lines (up to eight times basal levels). It is tempting to associate the radiation-enhanced VEGF secretion with an increased angiogenic potential of the tumor, which may be a factor in radioresistance.

Keywords

brain tumor glioblastoma multiforme ionizing radiation radioresistance vascular endothelial growth factor VEGF 

Copyright information

© Springer 2005

Authors and Affiliations

  • K. E. Hovinga
    • 1
    • 2
  • L. J. A. Stalpers
    • 1
  • C. van Bree
    • 1
  • M. Donker
    • 1
  • J. J. C. Verhoeff
    • 1
  • H. M. Rodermond
    • 1
  • D. A. Bosch
    • 2
  • W. R. van Furth
    • 2
  1. 1.Department of RadiotherapyUniversity of AmsterdamAmsterdamThe Netherlands
  2. 2.Department of Neurosurgery, Academic Medical Centre (AMC) H2-209University of AmsterdamDD AmsterdamThe Netherlands

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