Journal of Neuro-Oncology

, Volume 72, Issue 1, pp 17–23

Antisense bcl-2 transfection up-regulates anti-apoptotic and anti-oxidant thioredoxin in neuroblastoma cells

  • Yiting Li
  • Zhaohui Lu
  • Fang Chen
  • Jian Guan
  • Liping Hu
  • Yanfeng Xu
  • Jie Chen
Laboratory Investigation

DOI: 10.1007/s11060-004-3116-x

Cite this article as:
Li, Y., Lu, Z., Chen, F. et al. J Neurooncol (2005) 72: 17. doi:10.1007/s11060-004-3116-x

Abstract

Antisense bcl-2 therapy combined with chemotherapy has been proved to be effective in various tumors. However, the role played by antisense bcl-2 therapy alone is not clear. In this study, we compared the apoptosis and the protein profiles of antisense bcl-2 transfected human neuroblastoma SK-N-MC cells to the control cells. Flow cytometric data indicated that antisense bcl-2 transfection did not lead to more extensive apoptosis in SK-N-MC cells (14.9 ± 3.8%) than the control cells (10.3 ± 2.3%). The above observation was confirmed by fluorescence microscopy using Hoechst 33258 staining. However, antisense bcl-2 induced changes in the expression of various proteins as shown by proteomic comparison, which included the up-regulation of the anti-apoptotic and anti-oxidant protein thioredoxin. By western blot validation, thioredoxin was found to be up-regulated by 2.9-folds with the corresponding down-regulation of Bcl-2 by 2.1-folds. The up-regulation of thioredoxin may be a compensating mechanism for cell survival in neuroblastoma when Bcl-2 expression is suppressed, and it may to some extent attenuate the effectiveness of antisense bcl-2 therapy.

Keywords

apoptosisneuroblastomaprotein c-bcl-2proteomeproto-oncogenethioredoxin

Copyright information

© Springer 2005

Authors and Affiliations

  • Yiting Li
    • 1
    • 3
  • Zhaohui Lu
    • 1
  • Fang Chen
    • 1
  • Jian Guan
    • 1
  • Liping Hu
    • 2
  • Yanfeng Xu
    • 1
  • Jie Chen
    • 1
  1. 1.Department of PathologyPeking Union Medical College HospitalBeijingP.R. China
  2. 2.National Laboratory of Medical Molecular Biology, Institute of Basic MedicineChinese Academy of Medical Sciences, Peking Union Medical CollegeBeijingP.R. China
  3. 3.Texas Children’s HospitalHoustonUSA