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Cobalt-doped nanohydroxyapatite: synthesis, characterization, antimicrobial and hemolytic studies

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Abstract

Hydroxyapatite (Ca10(PO4)6(OH)2; HAP) is a major mineral component of the calcified tissues, and it has various applications in medicine and dentistry. In the present investigation, cobalt-doped hydroxyapatite (Co-HAP) nanoparticles were synthesized by surfactant-mediated approach and characterized by different techniques. The EDAX was carried out to estimate the amount of doping in Co-HAP. The transmission electron microscopy result suggested the transformation of morphology from needle shaped to spherical type on increasing the doping concentration. The powder XRD study indicated the formation of a new phase of brushite for higher concentration of cobalt. The average particle size and strain were calculated using Williamson–Hall analysis. The average particle size was found to be 30–60 nm. The FTIR study confirmed the presence of various functional groups in the samples. The antimicrobial activity was evaluated against four organisms Pseudomonas aeruginosa and Shigella flexneri as Gram negative as well as Micrococcus luteus and Staphylococcus aureus as Gram positive. The hemolytic test result suggested that all samples were non-hemolytic. The photoluminescence study was carried out to identify its possible applicability as a fluorescent probe.

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Acknowledgments

The authors are thankful to UGC, New Delhi, for funding under SAP-DRS and Dr. K. V. R Murthy and Dr. Y. S. Gandhi (Applied Physics Department, M. S. University of Baroda) for luminescence data. The authors also thank Prof. H. H. Joshi and Prof. S. P. Singh for their keen interest. The authors are thankful to Dr. A.D.B. Vaidya for useful guidance and discussion. The author (KPT) is thankful to UGC, New Delhi, for SRF under Meritorious Students Scheme.

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Authors and Affiliations

  1. Crystal Growth Laboratory, Physics Department, Saurashtra University, Rajkot, 360 005, Gujarat, India

    Kashmira P. Tank & Mihir J. Joshi

  2. Bioscience Department, Saurashtra University, Rajkot, 360 005, Gujarat, India

    Kiran S. Chudasama & Vrinda S. Thaker

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  1. Kashmira P. Tank
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  2. Kiran S. Chudasama
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  3. Vrinda S. Thaker
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  4. Mihir J. Joshi
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Correspondence to Kashmira P. Tank or Mihir J. Joshi.

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Tank, K.P., Chudasama, K.S., Thaker, V.S. et al. Cobalt-doped nanohydroxyapatite: synthesis, characterization, antimicrobial and hemolytic studies . J Nanopart Res 15, 1644 (2013). https://doi.org/10.1007/s11051-013-1644-z

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