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Ciprofloxacin nano-niosomes for targeting intracellular infections: an in vitro evaluation

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Abstract

In order to propose non-ionic surfactant vesicles (niosomes) for the treatment of intracellular infections, a remote loading method (active drug encapsulation) followed by sonication was used to prepare nano-niosome formulations containing ciprofloxacin (CPFX). Size analysis, size distribution and zeta potentials of niosomes were evaluated and then their antimicrobial activity, cellular uptake, cytotoxicity, intracellular distribution, and antibacterial activity against intracellular Staphylococcus aureus infection of murine macrophage-like, J774, cells were investigated in comparison to free drug. Our findings reveal that size and composition of the niosome formula can influence their in vitro biological properties. Vesicles in the 300–600 nm size range were phagocytosed to a greater degree by macrophages in comparison to other size vesicles. The minimum inhibitory concentrations (MICs) of CPFX-loaded niosomes were two to eightfold lower than MICs of free CPFX. In addition, niosome encapsulation of CPFX provided high intracellular antimicrobial activities while free CPFX is ineffective for eradicating intracellular forms of S. aureus. Encapsulation of CPFX in niosomes generally decreased its in vitro cytotoxicity. Our results show that niosomes are suitable drug delivery systems with good efficacy and safety properties to be proposed for drug targeting against intracellular infections.

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Acknowledgments

This study was financially supported by Isfahan University of Medical Sciences (Grant No. 188097).

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Authors and Affiliations

  1. Department of Pharmaceutical Biotechnology and Isfahan Pharmaceutical Research Center, Faculty of Pharmacy, Isfahan University of Medical Sciences, Isfahan, Iran

    Vajihe Akbari, Daryoush Abedi & Hojjat Sadeghi-Aliabadi

  2. Pharmaceutics Research Center, Kerman University of Medical Sciences, Kerman, Iran

    Abbas Pardakhty

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  1. Vajihe Akbari
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  2. Daryoush Abedi
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  3. Abbas Pardakhty
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  4. Hojjat Sadeghi-Aliabadi
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Correspondence to Hojjat Sadeghi-Aliabadi.

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Akbari, V., Abedi, D., Pardakhty, A. et al. Ciprofloxacin nano-niosomes for targeting intracellular infections: an in vitro evaluation. J Nanopart Res 15, 1556 (2013). https://doi.org/10.1007/s11051-013-1556-y

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  • DOI: https://doi.org/10.1007/s11051-013-1556-y

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