Methotrexate-conjugated magnetic nanoparticles for thermochemotherapy and magnetic resonance imaging of tumor

  • Fuping Gao
  • Zixing Yan
  • Jing Zhou
  • Yuanyuan Cai
  • Jintian Tang
Research Paper

DOI: 10.1007/s11051-012-1160-6

Cite this article as:
Gao, F., Yan, Z., Zhou, J. et al. J Nanopart Res (2012) 14: 1160. doi:10.1007/s11051-012-1160-6

Abstract

There is significant interest in recent years in developing magnetic nanoparticles (MNPs) having multifunctional characteristics with complimentary roles. In this study, methotrexate (MTX) was conjugated on the iron oxide magnetic nanoparticles surface via a poly(ethyleneimine) self-assembled monolayer (MTX–MNPs). The novel platform combined cancer chemotherapy, hyperthermia and potential monitoring of the progression of disease through magnetic resonance imaging (MRI). The conjugation of MTX on the magnetite surface was confirmed by Fourier transform infrared spectroscopy and change of zeta potential. Transmission electron microscope (TEM) showed that MTX–MNPs were morphologically spherical. The average diameter of MTX–MNPs was 30.1 ± 5.2 nm determined by dynamic light scattering. Magnetic measurements revealed that the saturation magnetization of MTX–MNPs reached 68.8 emu/g and the nanoparticles were superparamagnetic. The MTX–MNPs had good heating properties in an alternating magnetic field. TEM results showed that a larger number of MTX–MNPs were internalized into the MCF-7 cellular cytoplasm compared with the MNPs. The MTX–MNPs demonstrated highly synergistic antiproliferative effects of simultaneous chemotherapy and hyperthermia in MCF-7 breast cancer cells. A significant negative contrast enhancement was observed with magnetic resonance phantom imaging for MCF-7 cells over L929cells, when both were cultured with the nanoconjugate. The MTX–MNPs with combined characteristics of thermochemotherapy and MRI could be of high clinical significance in the treatment of tumor.

Keywords

Magnetic nanoparticlesMethotrexateThermochemotherapyMagnetic resonance imagingMultifunction

Copyright information

© Springer Science+Business Media B.V. 2012

Authors and Affiliations

  • Fuping Gao
    • 1
  • Zixing Yan
    • 2
  • Jing Zhou
    • 1
  • Yuanyuan Cai
    • 1
  • Jintian Tang
    • 1
  1. 1.Key Laboratory of Particle & Radiation Imaging, Ministry of Education, Institute of Medical Physics and Engineering, Department of Engineering PhysicsTsinghua UniversityBeijingChina
  2. 2.Second HospitalShanxi Medical UniversityTaiyuanChina