Research Paper

Journal of Nanoparticle Research

, 14:1160

Methotrexate-conjugated magnetic nanoparticles for thermochemotherapy and magnetic resonance imaging of tumor

  • Fuping GaoAffiliated withKey Laboratory of Particle & Radiation Imaging, Ministry of Education, Institute of Medical Physics and Engineering, Department of Engineering Physics, Tsinghua University
  • , Zixing YanAffiliated withSecond Hospital, Shanxi Medical University
  • , Jing ZhouAffiliated withKey Laboratory of Particle & Radiation Imaging, Ministry of Education, Institute of Medical Physics and Engineering, Department of Engineering Physics, Tsinghua University
  • , Yuanyuan CaiAffiliated withKey Laboratory of Particle & Radiation Imaging, Ministry of Education, Institute of Medical Physics and Engineering, Department of Engineering Physics, Tsinghua University
  • , Jintian TangAffiliated withKey Laboratory of Particle & Radiation Imaging, Ministry of Education, Institute of Medical Physics and Engineering, Department of Engineering Physics, Tsinghua University Email author 

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Abstract

There is significant interest in recent years in developing magnetic nanoparticles (MNPs) having multifunctional characteristics with complimentary roles. In this study, methotrexate (MTX) was conjugated on the iron oxide magnetic nanoparticles surface via a poly(ethyleneimine) self-assembled monolayer (MTX–MNPs). The novel platform combined cancer chemotherapy, hyperthermia and potential monitoring of the progression of disease through magnetic resonance imaging (MRI). The conjugation of MTX on the magnetite surface was confirmed by Fourier transform infrared spectroscopy and change of zeta potential. Transmission electron microscope (TEM) showed that MTX–MNPs were morphologically spherical. The average diameter of MTX–MNPs was 30.1 ± 5.2 nm determined by dynamic light scattering. Magnetic measurements revealed that the saturation magnetization of MTX–MNPs reached 68.8 emu/g and the nanoparticles were superparamagnetic. The MTX–MNPs had good heating properties in an alternating magnetic field. TEM results showed that a larger number of MTX–MNPs were internalized into the MCF-7 cellular cytoplasm compared with the MNPs. The MTX–MNPs demonstrated highly synergistic antiproliferative effects of simultaneous chemotherapy and hyperthermia in MCF-7 breast cancer cells. A significant negative contrast enhancement was observed with magnetic resonance phantom imaging for MCF-7 cells over L929cells, when both were cultured with the nanoconjugate. The MTX–MNPs with combined characteristics of thermochemotherapy and MRI could be of high clinical significance in the treatment of tumor.

Keywords

Magnetic nanoparticles Methotrexate Thermochemotherapy Magnetic resonance imaging Multifunction