Journal of Nanoparticle Research

, Volume 13, Issue 10, pp 4717–4729

Multifunctional iron platinum stealth immunomicelles: targeted detection of human prostate cancer cells using both fluorescence and magnetic resonance imaging

  • Robert M. Taylor
  • Dale L. Huber
  • Todd C. Monson
  • Abdul-Mehdi S. Ali
  • Marco Bisoffi
  • Laurel O. Sillerud
Research Paper

DOI: 10.1007/s11051-011-0439-3

Cite this article as:
Taylor, R.M., Huber, D.L., Monson, T.C. et al. J Nanopart Res (2011) 13: 4717. doi:10.1007/s11051-011-0439-3

Abstract

Superparamagnetic iron oxide nanoparticles (SPIONs) are the most common type of contrast agents used in contrast agent-enhanced magnetic resonance imaging (MRI). Still, there is a great deal of room for improvement, and nanoparticles with increased MRI relaxivities are needed to increase the contrast enhancement in MRI applied to various medical conditions including cancer. We report the synthesis of superparamagnetic iron platinum nanoparticles (SIPPs) and subsequent encapsulation using PEGylated phospholipids to create stealth immunomicelles (DSPE-SIPPs) that can be specifically targeted to human prostate cancer cell lines and detected using both MRI and fluorescence imaging. SIPP cores and DSPE-SIPPs were 8.5 ± 1.6 nm and 42.9 ± 8.2 nm in diameter, respectively, and the SIPPs had a magnetic moment of 120 A m2/kg iron. J591, a monoclonal antibody against prostate specific membrane antigen (PSMA), was conjugated to the DSPE-SIPPs (J591-DSPE-SIPPs), and specific targeting of J591-DSPE-SIPPs to PSMA-expressing human prostate cancer cell lines was demonstrated using fluorescence confocal microscopy. The transverse relaxivity of the DSPE-SIPPs, measured at 4.7 Tesla, was 300.6 ± 8.5 s−1 mM−1, which is 13-fold better than commercially available SPIONs (23.8 ± 6.9 s−1 mM−1) and ~3-fold better than reported relaxivities for Feridex® and Resovist®. Our data suggest that J591-DSPE-SIPPs specifically target human prostate cancer cells in vitro, are superior contrast agents in T2-weighted MRI, and can be detected using fluorescence imaging. To our knowledge, this is the first report on the synthesis of multifunctional SIPP micelles and using SIPPs for the specific detection of prostate cancer.

Keywords

Iron platinumMicelleProstate cancerContrast agentMagnetic resonance imagingFluorescent nanoparticleNanomedicine

Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • Robert M. Taylor
    • 1
  • Dale L. Huber
    • 2
  • Todd C. Monson
    • 3
  • Abdul-Mehdi S. Ali
    • 4
  • Marco Bisoffi
    • 1
  • Laurel O. Sillerud
    • 1
  1. 1.Department of Biochemistry and Molecular BiologyUniversity of New Mexico School of MedicineAlbuquerqueUSA
  2. 2.Center for Integrated NanotechnologiesSandia National LaboratoriesAlbuquerqueUSA
  3. 3.Nanomaterials Sciences DepartmentSandia National LaboratoriesAlbuquerqueUSA
  4. 4.Department of Earth and Planetary SciencesUniversity of New MexicoAlbuquerqueUSA