Journal of Nanoparticle Research

, Volume 13, Issue 12, pp 6439–6445

Gold nanoparticles produced in a microalga

  • Tiyaporn Luangpipat
  • Isabel R. Beattie
  • Yusuf Chisti
  • Richard G. Haverkamp
Research Paper

DOI: 10.1007/s11051-011-0397-9

Cite this article as:
Luangpipat, T., Beattie, I.R., Chisti, Y. et al. J Nanopart Res (2011) 13: 6439. doi:10.1007/s11051-011-0397-9

Abstract

An efficient biological route to production of gold nanoparticles which allows the nanoparticles to be easily recovered remains elusive. Live cells of the green microalga Chlorella vulgaris were incubated with a solution of gold chloride and harvested by centrifugation. Nanoparticles inside intact cells were identified by transmission electron microscopy and confirmed to be metallic gold by synchrotron based X-ray powder diffraction and X-ray absorption spectroscopy. These intracellular gold nanoparticles were 40–60 nm in diameter. At a concentration of 1.4% Au in the alga, a better than 97% recovery of the gold from solution was achieved. A maximum of 4.2% Au in the alga was obtained. Exposure of C. vulgaris to solutions containing dissolved salts of palladium, ruthenium, and rhodium also resulted in the production of the corresponding nanoparticles within the cells. These were surmised to be also metallic, but were produced at a much lower intracellular concentration than achieved with gold. Iridium was apparently toxic to the alga. No nanoparticles were observed using platinum solutions. C. vulgaris provides a possible route to large scale production of gold nanoparticles.

Keywords

NanoparticlesChlorella vulgarisGoldPlatinumPalladiumIridiumRutheniumRhodiumNanobiotechnology

Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • Tiyaporn Luangpipat
    • 1
  • Isabel R. Beattie
    • 1
  • Yusuf Chisti
    • 1
  • Richard G. Haverkamp
    • 1
  1. 1.School of Engineering and Advanced TechnologyMassey UniversityPalmerston NorthNew Zealand