Journal of Nanoparticle Research

, Volume 13, Issue 6, pp 2361–2374

Preparation of functional spherical polysilsesquioxane/gold nanoparticle composites and their applications in DNA assay

  • Jung A. Jung
  • Young Baek Kim
  • Young A. Kim
  • Seung Bum Ryu
  • Veronica Kim
Research paper

DOI: 10.1007/s11051-010-9997-z

Cite this article as:
Jung, J.A., Kim, Y.B., Kim, Y.A. et al. J Nanopart Res (2011) 13: 2361. doi:10.1007/s11051-010-9997-z
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Abstract

Functional spherical solid and hollow particles of polysilsesquioxanes (PSQs) containing amine, thiol, and vinyl groups were prepared by polymerizing organotrialkoxysilanes (OTASs) containing corresponding chemical groups. Fluorescent PSQ particles were prepared by physically entrapping Rhodamine 6G, Coumarin 7, and Fluoresceine sodium salts. The intensity of fluorescent light increased initially with increasing amount of entrapped fluorophores and then leveled off or decreased slightly after reaching a maximum value. PSQ particles containing gold nanoparticles (GNPs), both inside and on the surface, were prepared by the in situ reduction of gold ions by the PSQ particles. When the reduction reaction was carried out for extended periods of time, the GNP that had formed inside the poly(3-mercaptopropyl)silsesquioxane (PMPSQ) and polyvinylsilsesequioxane (PVSQ) particles underwent interesting morphological changes. PSQ particles containing amine and thiol groups fixed the GNPs on the surface, which could be utilized further in binding amine-modified oligo-DNA strands. The aggregation of PSQ/GNP particles combined with complementary oligo-DNA strands was examined to demonstrate that these particles could be applied to DNA assays and isolation. The particles were characterized by scanning electron microscopy, transmission electron microscopy, solid state nuclear magnetic resonance spectroscopy, ultraviolet/visible spectroscopy, and fluorescence microscopy.

Keywords

Fluorescent particles Polysilsesquioxane particles Gold nanoparticles DNA assay DNA–amine interaction Synthesis 

Supplementary material

11051_2010_9997_MOESM1_ESM.pptx (20.6 mb)
Supplementary material 1 (PPTX 21103 kb)

Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • Jung A. Jung
    • 1
  • Young Baek Kim
    • 1
  • Young A. Kim
    • 2
  • Seung Bum Ryu
    • 3
  • Veronica Kim
    • 4
  1. 1.Department of NanotechnologyPaiChai UniversityDaejeonKorea
  2. 2.PolyChrom IncAhnsanKorea
  3. 3.Alteogen, IncDaejeonKorea
  4. 4.Department of Animal ScienceCornell UniversityIthacaUSA

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