Journal of Nanoparticle Research

, Volume 13, Issue 6, pp 2461–2467

Detection of carcinoembryonic antigen using functional magnetic and fluorescent nanoparticles in magnetic separators

Research Paper

DOI: 10.1007/s11051-010-0138-5

Cite this article as:
Tsai, H.Y., Chang, C.Y., Li, Y.C. et al. J Nanopart Res (2011) 13: 2461. doi:10.1007/s11051-010-0138-5


We combined a sandwich immunoassay, anti-CEA/CEA/anti-CEA, with functional magnetic (~80 nm) and fluorescent (~180 nm) nanoparticles in magnetic separators to demonstrate a detection method for carcinoembryonic antigen (CEA). Determination of CEA in serum can be used in clinical diagnosis and monitoring of tumor-related diseases. The CEA concentrations in samples were deduced and determined based on the reference plot using the measured fluorescent intensity of sandwich nanoparticles from the sample. The linear range of CEA detection was from 18 ng/mL to 1.8 pg/mL. The detection limit of CEA was 1.8 pg/mL. In comparison with most other detection methods, this method had advantages of lower detection limit and wider linear range. The recovery was higher than 94%. The CEA concentrations of two serum samples were determined to be 9.0 and 55 ng/mL, which differed by 6.7% (9.6 ng/mL) and 9.1% (50 ng/mL) from the measurements of enzyme-linked immunosorbent assay (ELISA), respectively. The analysis time can be reduced to one third of ELISA. This method has good potential for other biomarker detections and biochemical applications.


ImmunoassayFunctional nanoparticlesMagnetic separatorDiagnosticsNanomedicine

Supplementary material

11051_2010_138_MOESM1_ESM.pdf (143 kb)
Supplementary material 1 (PDF 143 kb)

Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  1. 1.Department of Applied ChemistryChung Shan Medical UniversityTaichung, 402Taiwan
  2. 2.Department of Applied ChemistryNational Chi Nan UniversityPuli, Nantou, 545Taiwan
  3. 3.Clinical LaboratoryChung Shan Medical University HospitalTaichung, 402Taiwan