Research Paper

Journal of Nanoparticle Research

, Volume 12, Issue 6, pp 2069-2079

First online:

Nanosensors having dipicolinic acid imprinted nanoshell for Bacillus cereus spores detection

  • Aytaç GültekinAffiliated withDepartment of Chemistry, Trakya University
  • , Arzu ErsözAffiliated withDepartment of Chemistry, Faculty of Science, Yunusemre Campus , Anadolu University
  • , Nalan Yılmaz SarıözlüAffiliated withDepartment of Biology, Anadolu University
  • , Adil DenizliAffiliated withDepartment of Chemistry, Hacettepe University
  • , Rıdvan SayAffiliated withDepartment of Chemistry, Faculty of Science, Yunusemre Campus , Anadolu UniversityBİBAM (Plant, Drug and Scientific Researchers Center), Anadolu University Email author 

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Abstract

Molecular imprinted polymers (MIPs) as a recognition element for sensors are increasingly of interest and MIP nanoclusters have started to appear in the literature. In this study, we have proposed a novel thiol ligand-capping method with polymerizable methacryloylamido-cysteine (MAC) attached to gold–silver nanoclusters, reminiscent of a self-assembled monolayer and have reconstructed surface shell by synthetic host polymers based on molecular imprinting method for recognition. In this method, methacryloylamidoantipyrine–terbium ((MAAP)2–Tb(III)) has been used as a new metal-chelating monomer via metal coordination–chelation interactions and dipicolinic acid (DPA) which is main participant of Bacillus cereus spores used as a model. Nanoshell sensors with templates give a cavity that is selective for DPA. The DPA can simultaneously chelate to Tb(III) metal ion and fit into the shape-selective cavity. Thus, the interaction between Tb(III) ion and free coordination spheres has an effect on the binding ability of the gold–silver nanoclusters nanosensor. The binding affinity of the DPA imprinted nanoclusters has been investigated by using the Langmuir and Scatchard methods, and the respective affinity constants (K affinity) determined were found to be 1.43 × 104 and 9.1 × 106 mol L−1.

Keywords

Gold–silver nanoclusters sensor Molecularly imprinted polymers Dipicolinic acid Bacillus cereus spores recognition Photoluminescence