Journal of Nanoparticle Research

, Volume 12, Issue 1, pp 55–60

Analysis of the toxicity of gold nano particles on the immune system: effect on dendritic cell functions

Authors

    • Inserm, U823, Centre de Recherche Albert Bonniot
    • Université Joseph Fourier
  • Heidi Freitas
    • Inserm, U823, Centre de Recherche Albert Bonniot
    • Université Joseph Fourier
  • Rachel Couderc
    • Inserm, U823, Centre de Recherche Albert Bonniot
    • Université Joseph Fourier
  • Marie-Bernadette Villiers
    • Inserm, U823, Centre de Recherche Albert Bonniot
    • Université Joseph Fourier
  • Patrice N. Marche
    • Inserm, U823, Centre de Recherche Albert Bonniot
    • Université Joseph Fourier
Special focus: Safety of Nanoparticles

DOI: 10.1007/s11051-009-9692-0

Cite this article as:
Villiers, C.L., Freitas, H., Couderc, R. et al. J Nanopart Res (2010) 12: 55. doi:10.1007/s11051-009-9692-0

Abstract

The effect of manufactured gold nanoparticles (NPs) on the immune system was analysed through their ability to perturb the functions of dendritic cells (DCs), a major actor of both innate and acquired immune responses. For this purpose, DCs were produced in culture from mouse bone marrow progenitors. The analysis of the viability of the cells after their incubation in the presence of gold NPs shows that these NPs are not cytotoxics even at high concentration. Furthermore, the phenotype of the DC is unchanged after the addition of NPs, indicating that there is no activation of the DC. However, the analysis of the cells at the intracellular level reveals important amounts of gold NPs amassing in endocytic compartments. Furthermore, the secretion of cytokines is significantly modified after such internalisation indicating a potential perturbation of the immune response.

Keywords

NanoparticlesGoldImmune systemDendritic cellsCytotoxicityCytokineNanomedicineEHS

Copyright information

© Springer Science+Business Media B.V. 2009