Journal of Nanoparticle Research

, Volume 11, Issue 5, pp 1219–1225

Facile method for CLSM imaging unfunctionalized Au nanoparticles through fluorescent channels

  • Lan Yuan
  • Wei Wei
  • Juan Li
  • Zhiwei Sun
  • Hongfang Wang
  • Xiuzhi Zhang
  • Yueyue Chen
Technology and Applications

DOI: 10.1007/s11051-009-9618-x

Cite this article as:
Yuan, L., Wei, W., Li, J. et al. J Nanopart Res (2009) 11: 1219. doi:10.1007/s11051-009-9618-x

Abstract

The microscopic visualization of metal nanoparticles has become a useful tool for the investigation of their applications in cell labeling and the study of their bio-effects. In the current study, we have developed a facile method with confocal laser scanning microscope (CLSM) to observe unfunctionalized Au nanoparticles through fluorescent channels. The sharp reflected signal and photostable property of the metal nanoparticles makes the present method very ideal for fluorescent co-localization, real-time imaging, and further quantitative analysis.

Keywords

Au nanoparticlesCLSMReal-time imagingInstrumentationNanomedicine

Supplementary material

11051_2009_9618_MOESM1_ESM.doc (1.6 mb)
(DOC 1627 kb)

Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • Lan Yuan
    • 1
  • Wei Wei
    • 2
  • Juan Li
    • 3
  • Zhiwei Sun
    • 3
  • Hongfang Wang
    • 3
  • Xiuzhi Zhang
    • 3
  • Yueyue Chen
    • 3
  1. 1.Medical and Healthy Analytical CenterPeking University Health Science CenterBeijingPeople’s Republic of China
  2. 2.Institute of Process EngineeringChinese Academy of SciencesBeijingPeople’s Republic of China
  3. 3.School of Public HealthJilin UniversityChangchunPeople’s Republic of China