Journal of Nanoparticle Research

, Volume 12, Issue 1, pp 151–160

Immunological impact of magnetic nanoparticles (Ferucarbotran) on murine peritoneal macrophages

Authors

  • Chen-Hao Yeh
    • Department of HorticultureNational Taiwan University
  • Jong-Kai Hsiao
    • Department of Medical ImagingNational Taiwan University Hospital and College of Medicine
  • Jaw-Lin Wang
    • Institute of Biomedical EngineeringNational Taiwan University
    • Department of HorticultureNational Taiwan University
    • Research Center of Food and BiomoleculesNational Taiwan University
Research Paper

DOI: 10.1007/s11051-009-9589-y

Cite this article as:
Yeh, C., Hsiao, J., Wang, J. et al. J Nanopart Res (2010) 12: 151. doi:10.1007/s11051-009-9589-y

Abstract

Ferucarbotran, a clinically used superparamagnetic iron oxide, is widely developed as a magnetic resonance imaging (MRI) contrast agent and has the potential to improve the monitoring of macrophage recirculation in vivo. However, the biological effect of Ferucarbotran or magnetic nanoparticles (MNPs) on macrophage is not clearly understood yet. This study is aimed to examine the immunological impact of Ferucarbotran toward murine peritoneal macrophages. Cells treated with Ferucarbotran demonstrated a dose–responsive increase of granularity in the cytoplasm. After 24 h of incubation, viability and cytotoxicity in macrophages treated with 200 μg Fe/mL of Ferucarbotran were not affected. Macrophages loaded with Ferucarbotran above 100 μg Fe/mL showed a significant (p < 0.01) increase in cytokine (TNF-α, IL-1β, IL-6) secretion and mRNA expression, followed by nitric oxide (NO) secretion and iNOS mRNA expression. Chemotactic responses of Ferucarbotran-preloaded macrophages toward CX3CL1 were significantly (p < 0.05) lower than those of untreated macrophages. Taking together, Ferucarbotran at high dose (100 μg Fe/mL) could induce murine peritoneal macrophages activation in pro-inflammatory cytokine secretion and NO production.

Keywords

Superparamagnetic iron oxideMagnetic resonance imagingCytotoxicityInflammationCell activationChemotaxisNanomedicine

Abbreviations

MNPs

Magnetic nanoparticles

MRI

Magnetic resonance imaging

SPIO

Superparamagnetic iron oxide

USPIO

Ultrasmall superparamagnetic iron oxide

Supplementary material

11051_2009_9589_MOESM1_ESM.doc (46 kb)
Supplementary material 1 (DOC 46 kb)

Copyright information

© Springer Science+Business Media B.V. 2009