Journal of Nanoparticle Research

, Volume 9, Issue 6, pp 1133–1138

Silver nanoparticles as matrix for laser desorption/ionization mass spectrometry of peptides

Technology and Applications

DOI: 10.1007/s11051-007-9244-4

Cite this article as:
Hua, L., Chen, J., Ge, L. et al. J Nanopart Res (2007) 9: 1133. doi:10.1007/s11051-007-9244-4


Silver nanoparticle synthesized from chemical reduction has been successfully utilized as a matrix in matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) of peptides. Acting as a substrate to adsorb analytes, as well as a transmission medium for UV laser, silver nanoparticle was found to assist in the desorption/ionization of peptides with little or no induced fragmentation. The size of the nanoparticle was typically in the range of 160 ± 20 nm. One of the key advantages of silver nanoparticle for peptides analysis is its simple step for on-probe sample preparation. In addition, it also minimizes the interferences of sodium dodecyl sulfate (SDS) surfactant background signal, resulting in cleaner mass spectra and more sensitive signal, when compared to α–cyano-4-hydroxycinnamic acid (CCA) matrix.


Silver nanoparticle Matrix-assisted laser Desorption/ionization Chemical reduction Mass spectrometry Peptides Nanocomposites Instrumentation 

Copyright information

© Springer Science+Business Media B.V. 2007

Authors and Affiliations

  • Lin Hua
    • 1
  • Jianrong Chen
    • 1
  • Liya Ge
    • 2
  • Swee Ngin Tan
    • 2
  1. 1.College of Chemistry and Life ScienceZhejiang Normal UniversityJinhuaP.R. China
  2. 2.Natural Sciences and Science Education Academic GroupNanyang Technological UniversitySingaporeSingapore