Natural Computing

, Volume 7, Issue 2, pp 287–298

Unravel four hairpins!

  • Atsushi Kameda
  • Masahito Yamamoto
  • Azuma Ohuchi
  • Satsuki Yaegashi
  • Masami Hagiya
Article

DOI: 10.1007/s11047-007-9035-y

Cite this article as:
Kameda, A., Yamamoto, M., Ohuchi, A. et al. Nat Comput (2008) 7: 287. doi:10.1007/s11047-007-9035-y

Abstract

DNA machines consisting of consecutive hairpins, which we have previously described, have various potential applications in DNA computation. In the present study, a 288-base DNA machine containing four consecutive hairpins was successfully constructed by ligation and PCR. PAGE and fluorescence spectroscopy experiments verified that all four hairpins were successfully opened by four opener oligomers, and that hairpin opening was dependent on the proper openers added in the correct order. Quantitative analysis of the final results by fluorescence spectroscopy indicated that all four hairpins were open in about 1/4 to 1/3 of the DNA machines.

Keywords

DNA computing DNA nanotechnology Molecular computing Molecular memory Molecular machine 

Copyright information

© Springer Science+Business Media, Inc. 2007

Authors and Affiliations

  • Atsushi Kameda
    • 1
  • Masahito Yamamoto
    • 1
    • 2
  • Azuma Ohuchi
    • 1
    • 2
  • Satsuki Yaegashi
    • 1
  • Masami Hagiya
    • 1
    • 3
  1. 1.Japan Science and Technology CorporationKawaguchi City, SaitamaJapan
  2. 2.Graduate School of Information Science and TechnologyHokkaido UniversityKita-ku, SapporoJapan
  3. 3.Department of Computer Science, Graduate School of Information Science and TechnologyUniversity of TokyoTokyoJapan

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