In Vitro Susceptibility of Environmental Isolates of Exophiala dermatitidis to Five Antifungal Drugs
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Several dematiaceous fungi frequently isolated from nature are involved in cases of superficial lesions to lethal cerebral infections. Antifungal susceptibility data on environmental and clinical isolates are still sparse despite the advances in testing methods. The objective of this study was to examine the activities of 5-flucytosine, amphotericin B, itraconazole, voriconazole and terbinafine against environmental isolates of Exophiala strains by minimum inhibition concentration (MIC) determination. The strains were obtained from hydrocarbon-contaminated soil, ant cuticle and fungal pellets from the infrabuccal pocket of attine gynes. Broth microdilution assay using M38-A2 reference methodology for the five antifungal drugs and DNA sequencing for fungal identification were applied. Terbinafine was the most active drug against the tested strains. It was observed that amphotericin B was less effective, notably against Exophiala spinifera, also studied. High MICs of 5-flucytosine against Exophiala dermatitidis occurred. This finding highlights the relevance of studies on the antifungal resistance of these potential opportunistic species. Our results also contribute to a future improvement of the standard methods to access the drug efficacy currently applied to black fungi.
- Badali H, Chander J, Bayat M, et al. Multiple subcutaneous cysts due to Exophiala spinifera in an immunocompetent patient. Med Mycol. 2012;50:207–13. CrossRef
- Badali H, De Hoog GS, Sudhadham M, Meiss JF. Microdilution in vitro antifungal susceptibility of Exophiala dermatitidis, a systemic opportunist. Med Mycol. 2011;49:819–24.
- Biancalana FS, Lyra L, Schreiber AZ. In vitro evaluation of the type of interaction obtained by the combination of terbinafine and itraconazole, voriconazole, or amphotericin B against dematiaceous molds. Antimicrob Agents Chemother. 2011;55:4485–7. CrossRef
- Brandt ME, Warnock DW. Epidemiology, clinical manifestations, and therapy of infections caused by dematiaceous fungi. J Chemother. 2003;15:36–47.
- Caligiorne RB, Resende MA, Melillo PH, et al. In vitro susceptibility of chromoblastomycosis and phaeohyphomycosis agents to antifungal drugs. Med Mycol. 1999;37:405–9. CrossRef
- Castro LGM, Andrade TS. Chromoblastomycosis: still a therapeutic challenge. Expert Rev Dermatol. 2010;5:433–43. CrossRef
- Chandrasekar PH, Manavathu E. Voriconazole: a second generation triazole. Drugs Today. 2001;37:135–48.
- Chen A, Sobel JD. Emerging azole antifungals. Expert Opin Emerg Drugs. 2005;10:21–33. CrossRef
- Chryssanthou E, Cuenca-Estrella M. Comparison of the antifungal susceptibility testing subcommittee of the European committee on antibiotic susceptibility testing proposed standard and the E-test with the NCCLS broth microdilution method for voriconazole and caspofungin susceptibility testing of yeast species. J Clin Microbiol. 2002;40:3841–4. CrossRef
- CLSI. Clinical Laboratory Standards Institute. Reference method for broth dilution antifungal susceptibility testing of filamentous fungi: second edition (M38–A2). Wayne: CLSI; 2008.
- Cuenca-Estrella M, Gomez-Lopez A, Mellado E, Buitrago MJ, Monzon A, Rodriguez-Tudela JL. Head-to-head comparison of the activities of currently available antifungal agents against 3,378 Spanish clinical isolates of yeasts and filamentous fungi. Antimicrob Agents Chemother. 2006;50:917–21. CrossRef
- Espinel-Ingroff A, Chaturvedi V, Fothergill A, Rinaldi MG. Optimal testing conditions for determining MICs and minimum fungicidal concentrations of new and established antifungal agents for uncommon molds: NCCLS collaborative study. J Clin Microbiol. 2002;40:3776–81. CrossRef
- Espinel-Ingroff A. In vitro fungicidal activities of voriconazole, itraconazole, and amphotericin B against opportunistic moniliaceous and dematiaceous fungi. J Clin Microbio. 2001;39:954–8. CrossRef
- Fothergill AW, Rinaldi MG, Sutton DA. Antifungal susceptibility testing of Exophiala spp.: a head-to-head comparison of amphotericin B, itraconazole, posaconazole and voriconazole. Med Mycol. 2009;47:41–3. CrossRef
- van den Gerrits Ende AHG, de Hoog GS. Variability and molecular diagnostics of the neurotropic species Cladophialophora bantiana. Stud Mycol. 1999;43:152–62.
- Hall TA. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser. 1999;41:95–8.
- Harris JE, Sutton DA, Rubin A, Wickes B, de Hoog GS, Kovarik C. Exophiala spinifera as a cause of cutaneous phaeohyphomycosis: case study and review of the literature. Med Mycol. 2009;47:87–93. CrossRef
- Krishnan-Natesan S. Terbinafine: a pharmacological and clinical review. Expert Opin Pharmacother. 2009;10:2723–33. CrossRef
- Li DM, Li RY, de Hoog GS, Sudhadham M, Wang DL. Fatal Exophiala infections in China, with a report of seven cases. Mycoses. 2011;54:e136–42. CrossRef
- Li D, Li R, Wang D, Ma S. In vitro activities of five antifungal agents against pathogenic Exophiala species. Chin Med J. 1999;112:484–8.
- Lin YP, Li W, Yang YP, Huang WM, Fan YM. Cutaneous phaeohyphomycosis caused by Exophiala spinifera in a patient with systemic lupus erythematosus. Lupus. 2011;21:548–51. CrossRef
- Masclaux F, Gueho E, de Hoog GS, Christen R. Phylogenetic relationship of human-pathogenic Cladosporium (Xylohypha) species inferred from partial LS rRNA sequences. J Med Vet Mycol. 1995;33:327–38. CrossRef
- McGinnis MR, Pasarell L. In vitro testing of susceptibilities of filamentous ascomycetes to voriconazole, itraconazole, and amphotericin, with consideration of phylogenetic implications. J Clin Microbiol. 1998;36:2353–5.
- Meletiadis J, Mouton JW, Rodriguez-Tudela JL, Meiss JF, Verweij PE. In vitro interaction of terbinafine with itraconazole against clinical isolates of Scedosporium prolificans. Antimicrob Agents Chemother. 2000;44:470–2. CrossRef
- Negroni R, Helou SH, Petri N, Robles AM, Arechavala A, Bianchi MH. Case study: posaconazole treatment of disseminated phaeohyphomycosis due to Exophiala spinifera. Clin Infect Dis. 2004;8:e15–20. CrossRef
- Padhye AA, Ajello L, Chandler FW, et al. Phaeohyphomycosis in El Salvador caused by Exophiala spinifera. Am J Trop Med Hyg. 1983;32:799–803.
- Padhye AA, Hampton AA, Hampton MT, et al. Chromoblastomycosis caused by Exophiala spinifera. Clin Infect Dis. 1996;22:331–5. CrossRef
- Pang KR, Wu JJ, Huang DB, Tyring SK. Subcutaneous fungal infections. Dermatol Ther. 2004;17:523–53. CrossRef
- Pfaller MA, Andes D, Arendrup MC, et al. Clinical breakpoints for voriconazole and Candida spp. revisited: review of microbiologic, molecular, pharmacodynamic, and clinical data as they pertain to the development of species-specific interpretive criteria. Diag Microbiol Infect Dis. 2011;70:330–43. CrossRef
- Pradinaud MA, Bolzinger T. Treatment of chromoblastomycosis. J Am Acad Dermatol. 1991;25:869–70. CrossRef
- Radhakrishnan D, Jayalakshmi G, Madhumathy A, Banu ST, Geethalakshmi S, Sumathi G. Subcutaneous phaeohyphomycosis due to Exophiala spinifera in an immunocompromised host. Indian J Med Microbiol. 2010;28:396–9. CrossRef
- Revankar SG. Dematiaceous fungi. Mycoses. 2007;50:91–101. CrossRef
- Revankar SG. Phaeohyphomycosis. Infect Dis Clin N Am. 2006;20:609–20. CrossRef
- Sabatelli F, Patel R, Mann PA, et al. In vitro activities of posaconazole, fluconazole, itraconazole, voriconazole, and amphotericin B against a large collection of clinically important molds and yeasts. Antimicrob Agents Chemother. 2006;50:2009–15. CrossRef
- Satow MM, Attili-Angelis D, de Hoog GS, de Angelis DF Vicente VA. Selective factors involved in oil flotation isolation of black yeasts from the environment. Stud Mycol. 2008;61:157–63. CrossRef
- Sudhadham M, de Hoog GS, Menken SB, Gerrits VDEA, Sihanonth P. Rapid screening for genotypes as possible markers of virulence in the neurotropic black yeast Exophiala dermatitidis using PCR-RFLP. J Microbiol Methods. 2010;80:138–42. CrossRef
- Liu Sun Yi Wei, Wan Zhe, Wang Xiaohong, Li Ruoyu. Antifungal activity of antifungal drugs, as well as drug combinations against Exophiala dermatitidis. Mycopathologia. 2011;171:111–7. CrossRef
- Uijthof JM, de Hoog GS, de Cock AW, Takeo K, Nishimura K. Pathogenicity of strains of the black yeast Exophiala (Wangiella) dermatitidis: an evaluation based on polymerase chain reaction. Mycoses. 1994;37:235–42. CrossRef
- Vicente VA, Attili-Angelis D, Pie MR, et al. Environmental isolation of black yeast-like fungi involved in human infection. Stud Mycol. 2008;61:137–44. CrossRef
- Vitale RG, Afeltra J, de Hoog GS, Rijs AJ, Verweij PE. In vitro activity of amphotericin B and itraconazole in combination with flucytosine, sulfadiazine and quinolones against Exophiala spinifera. J Antimicrobial Chemother. 2003;51:1297–300. CrossRef
- Vitale RG, de Hoog GS, Verweij PE. In vitro activity of amphotericin, itraconazole, terbinafine and 5-flucytosine against Exophiala spinifera and evaluation of post antifungal effects. Med Mycol. 2003;41:301–7. CrossRef
- Vitale RG, de Hoog GS. Molecular diversity, new species and antifungal susceptibilities in the Exophiala spinifera clade. Med Mycol. 2002;40:545–56.
- White TJ, Bruns T, Lee S, Taylor JW. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH, Sninsky JJ, White TJ, editors. PCR protocols: a guide to methods and applications. New York: Academic Press; 1990. p. 315–22.
- Zeng JS, Sutton DA, Fothergill AW, Rinaldi MG, Harrak MJ, de Hoog GS. Spectrum of clinically relevant Exophiala species in the United States. J Clin Microbiol. 2007;45:3713–20. CrossRef
- Zhang J, Xi L, Lu C, Li X, Xie T, Zhang H, Xie Z, de Hoog S. Successful treatment for chromoblastomycosis caused by Fonsecaea monophora: a report of three cases in Guangdong. China Mycoses. 2009;52:176–81. CrossRef
- In Vitro Susceptibility of Environmental Isolates of Exophiala dermatitidis to Five Antifungal Drugs
Volume 175, Issue 5-6 , pp 455-461
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- Author Affiliations
- 1. Center for the Study of Social Insects, IB, UNESP- São Paulo State University, Rio Claro, SP, Brazil
- 2. Department of Biochemistry and Microbiology, IB, UNESP-São Paulo State University, Av. 24A, n. 1515, Bela Vista, Rio Claro, SP, 13506-900, Brazil
- 4. Adolfo Lutz Public Health Reference Laboratory, Rio Claro, SP, Brazil
- 3. Division of Microbial Resources, CPQBA, University of Campinas, Campinas, SP, Brazil