Mycopathologia

, 166:285

Secreted Proteases from Dermatophytes

Article

DOI: 10.1007/s11046-008-9105-4

Cite this article as:
Monod, M. Mycopathologia (2008) 166: 285. doi:10.1007/s11046-008-9105-4

Abstract

Dermatophytes are highly specialized pathogenic fungi that exclusively infect the stratum corneum, nails or hair, and it is evident that secreted proteolytic activity is important for their virulence. Endo- and exoproteases-secreted by dermatophytes are similar to those of species of the genus Aspergillus. However, in contrast to Aspergillus spp., dermatophyte-secreted endoproteases are multiple and are members of two large protein families, the subtilisins (serine proteases) and the fungalysins (metalloproteases). In addition, dermatophytes excrete sulphite as a reducing agent. In the presence of sulphite, disulphide bounds of the keratin substrate are directly cleaved to cysteine and S-sulphocysteine, and reduced proteins become accessible for further digestion by various endo- and exoproteases secreted by the fungi. Sulphitolysis is likely to be an essential step in the digestion of compact keratinized tissues which precedes the action of all proteases.

Keywords

AspergillusDermatophytesMicrosporumSecreted proteasesTrichophyton

Abbreviations

AMC

7-Amido-4-methylcoumarin

Dpp

Dipeptidyl peptidase

DTH

Delayed-type hypersensitivity

IH

Immediate hypersensitivity

Lap

Leucine aminopeptidase

Mcp

Metallocarboxypeptidase

Mep

Metalloprotease

MM

Molecular mass

MS

Mass spectrometry

pNA

p-Nitroanilide

PMSF

Phenyl methyl sulphonyl fluoride

Scp

Serine carboxypeptidase

Sub

Subtilisin

Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  1. 1.Service de Dermatologie et Vénéréologie, Laboratoire de Mycologie, BT422Centre Hospitalier Universitaire VaudoisLausanneSwitzerland