Mycopathologia

, Volume 165, Issue 1, pp 5–12

Effect of pre-incubation temperature on susceptibility of Galleria mellonella larvae to infection by Candida albicans

Authors

  • Peter Mowlds
    • Medical Mycology Unit, NICB, Department of BiologyNational University of Ireland Maynooth
    • Medical Mycology Unit, NICB, Department of BiologyNational University of Ireland Maynooth
Article

DOI: 10.1007/s11046-007-9069-9

Cite this article as:
Mowlds, P. & Kavanagh, K. Mycopathologia (2008) 165: 5. doi:10.1007/s11046-007-9069-9

Abstract

The use of insects for evaluating the virulence of microbial pathogens and for determining the efficacy of antimicrobial drugs is increasing. When larvae of the greater wax moth Galleria mellonella were incubated at 4 or 37°C for 24 h. prior to infection, they manifested increased resistance to infection by the yeast Candida albicans compared to larvae that had been pre-incubated for 24 h at 30°C. Incubation at 4 or 37°C led to an increase in haemocyte density and the expression of genes coding for gallerimycin, transferrin, an inducible metalloproteinase inhibitor (IMPI) and galiomicin. Peak expression of these genes was recorded at approximately 24 h after the commencement of the 4 or 37°C incubation. These results indicate that exposure of larvae to mild thermal shock conditions induces a protective cellular and humoral immune response mediated by increased numbers of haemocytes and elevated expression of antimicrobial peptides.

Keywords

Anti microbial peptidesCellular responseGalleria mellonellaGene expressionHaemocytesHost defenceHumoral response

Abbreviations

AMP

Anti-microbial peptide(s)

IMPI

Inducible metalloproteinase inhibitor

Copyright information

© Springer Science+Business Media B.V. 2007