, Volume 160, Issue 2, pp 137-142

First online:

Blastomyces Dermatitidis Antigen Detection in Urine Specimens from Dogs with Blastomycosis Using a Competitive Binding Inhibition ELISA

  • J.F. ShurleyAffiliated withDepartment of Biological Sciences, Idaho State University
  • , A.M. LegendreAffiliated withUniversity of Tennessee College of Veterinary Medicine
  • , G.M. ScalaroneAffiliated withDepartment of Biological Sciences, Idaho State University Email author 

Rent the article at a discount

Rent now

* Final gross prices may vary according to local VAT.

Get Access


A competitive binding inhibition enzyme linked immunosorbent assay (ELISA) was used to detect Blastomyces dermatitidis antigens in urine specimens from dogs with blastomycosis. Sera from rabbits immunized with B. dermatitidis killed whole yeast cells were used as the primary antibody in the competitive ELISA. This initial study was performed to determine if B. dermatitidis antigen detection was possible and to test the efficacy of the rabbit sera as a primary antibody. An indirect ELISA was also performed to compare antigen detection in urine to antibody detection in the sera of the infected dogs. The results indicate 100% (36/36 specimens) detection of both antigen and antibody. Cross reactivity with Histoplasma capsulatum, as well as non-specific binding with the normal urine specimens, was observed with the competitive binding inhibition ELISA.


antigen detection Blastomyces dermatitidis blastomycosis competitive ELISA urine