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UBXN1 polymorphism and its expression in porcine M. longissimus dorsi are associated with water holding capacity

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Abstract

The UBX domain containing protein 1-like gene (UBXN1) promotes the protein degradation that affects meat quality, in particular traits related to water holding capacity. The aim of our study was to identify UBXN1 polymorphisms and to analyse their association with meat quality traits. Moreover, the relationship of UBXN1 polymorphisms and its transcript abundance as well as the link between UBXN1 expression and water holding capacity were addressed. Pigs of the breed German landrace (GL) and the commercial crossbreed of Pietrain × [German large white × GL] (PiF1) were used for this study. In GL, the novel SNP c.355 C > T showed significant association with conductivity and drip loss (P ≤ 0.05). Another SNP at nt 674 of the coding sequence [SNP c.674C>T (p.Thr225Ile)] was associated with drip loss (P ≤ 0.05) and pH1 (P ≤ 0.1). In PiF1, the SNP UBXN1 c.674C>T was associated with conductivity (P ≤ 0.01). Moreover, the haplotype combinations showed effects on conductivity within both commercial populations at P ≤ 0.1. In both populations, high expression of UBXN1 tended to decrease water holding capacity in the early post mortem period. The analysis of triangular relationship of UBXN1 polymorphism, transcript abundance, and water holding capacity evidences the existence of a causal polymorphism in cis-regulatory regions of UBXN1 that influences its expression.

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Acknowledgments

This research was partly supported by the German Research Foundation (Deutsche Forschungsgemeinschaft, DFG; WI 1754/14-1) the Ministry of Education and Training of Vietnam, the German Academic Exchange Service (DAAD), and the Cantho University (Vietnam). Thanks are also due to the Pig Breeding Association of Mecklenburg-Western Pommerania.

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Authors and Affiliations

  1. Institute for Genome Biology, Leibniz Institute for Farm Animal Biology, Wilhelm-Stahl-Allee 2, 18196, Dummerstorf, Germany

    Huynh Thi Phuong Loan, Eduard Muráni & Klaus Wimmers

  2. Institute for Muscle Biology, Leibniz Institute for Farm Animal Biology, Wilhelm-Stahl-Allee 2, 18196, Dummerstorf, Germany

    Steffen Maak

  3. Research Group ‘Functional Genome Analysis’, Leibniz Institute for Farm Animal Biology, Wilhelm-Stahl-Allee 2, 18196, Dummerstorf, Germany

    Siriluck Ponsuksili

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  1. Huynh Thi Phuong Loan
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Correspondence to Klaus Wimmers.

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Loan, H.T.P., Muráni, E., Maak, S. et al. UBXN1 polymorphism and its expression in porcine M. longissimus dorsi are associated with water holding capacity. Mol Biol Rep 41, 1411–1418 (2014). https://doi.org/10.1007/s11033-013-2985-5

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  • DOI: https://doi.org/10.1007/s11033-013-2985-5

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