Molecular Biology Reports

, Volume 40, Issue 12, pp 6737–6745

Efficient growth inhibition of EGFR over-expressing tumor cells by an anti-EGFR nanobody

  • Kobra Omidfar
  • Fatemeh Sadat Amjad Zanjani
  • Arghavan Golbaz Hagh
  • Maedeh Darziani Azizi
  • Seyed Javad Rasouli
  • Susan Kashanian
Article

DOI: 10.1007/s11033-013-2790-1

Cite this article as:
Omidfar, K., Amjad Zanjani, F.S., Hagh, A.G. et al. Mol Biol Rep (2013) 40: 6737. doi:10.1007/s11033-013-2790-1

Abstract

Epidermal growth factor receptor (EGFR) is deemed to be one of the main molecular targets for diagnosis and treatment of cancer. It has been identified that EGFR involves in pathogenesis of some forms of human cancers. Monoclonal antibodies targeting EGFR could control the tumor cell growth, proliferation, and apoptosis by suppressing the signal transduction pathways. Nanobodies can be regarded as the smallest intact antigen binding fragments, derived from heavy chain-only antibodies existing in camelids. Here, we describe the identification of an EGFR-specific nanobody, referred to as OA-cb6, obtained from immunized camel with a cell line expressing high levels of EGFR. Utilizing flow cytometry (FACS) and blotting methods, we demonstrated that OA-cb6 nanobody binds specifically to EGFR expressing on the surface of A431 cells. In addition, OA-cb6 nanobody potently causes the inhibition of EGFR over expression, cell growth and proliferation. The antibody fragments can probably be regarded as worthwhile binding block for further rational design of anti-cancer therapy.

Keywords

EGFRNanobodyGrowth inhibitionTargeted therapy

Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • Kobra Omidfar
    • 1
    • 2
  • Fatemeh Sadat Amjad Zanjani
    • 1
  • Arghavan Golbaz Hagh
    • 1
  • Maedeh Darziani Azizi
    • 1
  • Seyed Javad Rasouli
    • 2
  • Susan Kashanian
    • 2
  1. 1.Biosensor Research Center, Endocrinology and Metabolism Molecular-Cellular Sciences InstituteTehran University of Medical SciencesTehranIran
  2. 2.Endocrinology and Metabolism Research Center, Endocrinology and Metabolism Research InstituteTehran University of Medical SciencesTehranIran